{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T11:11:17Z","timestamp":1788779477822,"version":"build-2803163510"},"reference-count":26,"publisher":"Wiley","issue":"20","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,10,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Chlorine gas is a highly toxic industrial chemical that poses a significant risk in accidental releases and deliberate terrorist attacks. Reliable analytical methods for the determination of chlorine in aqueous samples are therefore required for forensic and environmental investigations. This study aimed to develop a rapid and selective mass spectrometry\u2010based method for chlorine determination following derivatization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Chlorine in aqueous samples was derivatized with methyl 2\u2010(3,4,5\u2010trimethoxyphenyl)acetate (MTMPA) at 50\u00b0C for 15\u2009min, yielding methyl 2\u2010(2\u2010chloro\u20103,4,5\u2010trimethoxyphenyl)acetate (Cl\u2010MTMPA). The derivatization product was analyzed by gas chromatography\u2013mass spectrometry (GC\u2013MS) and liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS). While LC\u2013MS\/MS analysis was performed directly, GC\u2013MS analysis required an additional liquid\u2013liquid extraction step. Reaction conditions were optimized to maximize derivatization efficiency.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The derivatization reaction provided a high yield of Cl\u2010MTMPA under the optimized conditions. GC\u2013MS and LC\u2013MS\/MS analyses produced a common major fragment ion at\n                      <jats:italic>m\/z<\/jats:italic>\n                      215.0. Further structural confirmation by liquid chromatography\u2013high\u2010resolution tandem mass spectrometry (LC\u2010HRMS\/MS) revealed a characteristic product ion at\n                      <jats:italic>m\/z<\/jats:italic>\n                      215.0473 generated from the precursor ion at\n                      <jats:italic>m\/z<\/jats:italic>\n                      275.0681. Calibration curves covering acute toxicologically relevant chlorine concentrations (5\u2013200\u2009mg\/L) showed excellent linearity, with correlation coefficients (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      ) exceeding 0.99.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A rapid and selective derivatization\u2010based method was developed in which the resulting chlorine derivative is readily analyzed by both GC\u2013MS and LC\u2013MS\/MS. The method affords reliable analyte identification through characteristic fragmentation and excellent quantitative performance, making it suitable for forensic toxicology and environmental investigations involving chlorine exposure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70151","type":"journal-article","created":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T08:54:49Z","timestamp":1785488089000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Dual GC\u2013MS and LC\u2013MS\/MS Analytical Strategy for Evaluating Free Chlorine Toxicity in Aqueous Samples"],"prefix":"10.1002","volume":"40","author":[{"given":"Avital","family":"Shifrovitch","sequence":"first","affiliation":[{"name":"Department of Analytical Chemistry Israel Institute for Biological Research (IIBR)  Ness Ziona Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adi","family":"Tzadok","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Israel Institute for Biological Research (IIBR)  Ness Ziona Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moran","family":"Madmon","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Israel Institute for Biological Research (IIBR)  Ness Ziona Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1398-5246","authenticated-orcid":false,"given":"Avi","family":"Weissberg","sequence":"additional","affiliation":[{"name":"Department of Analytical Chemistry Israel Institute for Biological Research (IIBR)  Ness Ziona Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,31]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"151","article-title":"Chlorine Gas: An Evolving Hazardous Material Threat and Unconventional Weapon","volume":"11","author":"Jones R.","year":"2010","journal-title":"Western Journal of Emergency Medicine"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/00258024211002737"},{"key":"e_1_2_9_4_1","unstructured":"Agency for Toxic Substances and Disease Registry (ATSDR) \u201cToxicological Profile for Chlorine \u201d Atlanta GA: U.S Atlanta GA U.S. Department of Health and Human Services Public Health Service. 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