{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T15:02:36Z","timestamp":1789743756029,"version":"4.0.1"},"reference-count":32,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Untargeted lipidomics is commonly performed at analytical flow rates, which consume more solvent and may require higher on\u2010column sample loads when sensitivity is limited by analyte abundance. Nano flow separations use lower flow rates and sample loads, reducing solvent consumption and facilitating improved electrospray ionization. We present here practical considerations for implementing a routine nano flow lipidomics workflow.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Bovine liver total lipid extract was spiked with SPLASH Lipidomix internal standards and analyzed by nano flow and high flow liquid chromatography coupled to a high\u2010resolution accurate mass Orbitrap\u2010based mass spectrometer. Full\u2010scan polarity switching was used for untargeted profiling, and the AcquireX Deep Scan workflow was applied to support data\u2010dependent MS\/MS acquisition in the complex matrix. A SPLASH dilution series was analyzed in triplicate injections to compare analytical response across on\u2010column loads between the two workflows.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>At 25\u2009ng on\u2010column, nano flow yielded 1266 total lipid annotations and 835 high\u2010quality annotations, compared with 919 total and 518 high\u2010quality annotations for high flow at 100\u2009ng. Unintentional fragmentation decreased under nano flow conditions by 21%\u201331% across the representative lipid species evaluated. The use of nano flow allowed for the detection of lower on\u2010column loads across several standards, extending the lower end of the response range by up to 40\u2010fold compared with high flow analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Nano flow lipidomics improved sensitivity for untargeted analysis by increasing the number of lipid annotations, reducing unintentional fragmentation, and extending the analytical response to lower on\u2010column loads. Together with practical guidance around sample preparation, injection volume, washing, and equilibration, these results support nano flow chromatography as a sensitive and reliable approach for sample\u2010limited untargeted lipidomics.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70175","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T14:04:48Z","timestamp":1789740288000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Implementation of Nano Flow Chromatography Coupled to Mass Spectrometry as a Reliable and Sensitive Discovery Lipidomics Platform"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2668-8048","authenticated-orcid":false,"given":"Ciara J.","family":"Sallowicz","sequence":"first","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9377-2285","authenticated-orcid":false,"given":"Thiago","family":"Mattos","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6522-5724","authenticated-orcid":false,"given":"Rahul R.","family":"Deshpande","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bashar","family":"Amer","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan S.","family":"Bird","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California 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