{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T03:40:27Z","timestamp":1792640427629,"version":"4.5.0"},"reference-count":50,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T00:00:00Z","timestamp":1792540800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004772","name":"Natural Science Foundation of Ningxia Province","doi-asserted-by":"publisher","award":["2024AAC03716"],"award-info":[{"award-number":["2024AAC03716"]}],"id":[{"id":"10.13039\/501100004772","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,12,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>This study aims to elucidate the chemical composition of Jianpixiaocuo Capsule using UPLC\u2010Q\u2010Exactive\u2010Orbitrap\u2010MS and to explore its active ingredients, targets, and mechanisms in the treatment of acne vulgaris associated with spleen\u2010stomach damp\u2010heat syndrome through network pharmacology, molecular docking, and animal model validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The study identified chemical constituents with UPLC\u2010Q\u2010Exactive\u2010Orbitrap\u2010MS and predicted active ingredients and targets using databases like Traditional Chinese Medicine Systems Pharmacology (TCMSP), HERB, SwissADME, and SwissTargetPrediction. Acne\u2010related targets were gathered from GeneCards, OMIM, and DisGeNET. Intersecting targets were used to build a protein\u2013protein interaction (PPI) network via STRING to find core targets, which underwent GO\/KEGG enrichment analysis and molecular docking with AutoDock Vina. An in\u00a0vivo rat model of acne with spleen\u2010stomach damp\u2010heat syndrome was created for validation using histological analysis, serum cytokine measurement, and skin lesion scoring.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mass spectrometry identified 648 components in 18 categories, mainly terpenoids, flavonoids, and phenolic acids. Analysis found 705 capsule targets and 2014 disease\u2010related targets, with 217 overlaps. Core targets TNF, IL6, MAPK1, AKT1, and CXCL8 are linked to inflammation, sebum metabolism, and keratinization, enriched in NF\u2010\u03baB, IL\u201017, PI3K\u2010Akt, and MAPK pathways. Quercetin and baicalin showed strong binding to these targets. In\u00a0vivo tests confirmed the capsule's effectiveness, reducing skin inflammation and TNF\u2010\n                      <jats:italic>\u03b1<\/jats:italic>\n                      , IL\u20106, IL\u20101\n                      <jats:italic>\u03b2<\/jats:italic>\n                      levels, and altering MAPK\/NF\u2010\u03baB protein expression.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The Jianpixiaocuo Capsule, rich in terpenoids and flavonoids, effectively treats acne vulgaris linked to spleen\u2010stomach damp\u2010heat syndrome by modulating inflammation and sebum metabolism through a multicomponent, multitarget approach.<\/jats:p>\n                  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