{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:49:59Z","timestamp":1791877799057,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2026,12,30]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Insomnia is a highly prevalent disorder with substantial health and socioeconomic impacts, for which traditional Chinese medicine is an important treatment option. Jieyu Anshen Granule (JYAS\u2010G) shows excellent clinical efficacy in insomnia treatment, but its active components, in\u00a0vivo migration characteristics and underlying mechanisms remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Two mouse insomnia models induced by p\u2010chlorophenylalanine (PCPA, chemical method) and sleep deprivation machine (SDM, physical method) were established respectively, and mice were administered with low and high doses of JYAS\u2010G, with open field test used to evaluate the pharmacodynamic effects. UHPLC\u2010Q\u2010Orbitrap\u2010MS combined with GNPS was applied to systematically identify the in\u00a0vitro components of JYAS\u2010G and its migration profiles in mouse plasma, liver, heart, prefrontal cortex, hippocampus, and hypothalamus.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The SDM model induced insomnia with manic\u2010like behaviors, which were significantly ameliorated by JYAS\u2010G, whereas the PCPA model produced no significant insomnia\u2010like phenotypes under the current conditions. In total, 89 in\u00a0vitro components and 43 blood\u2010absorbed components of JYAS\u2010G were identified, showing marked tissue\u2010specific distribution. Network pharmacology and molecular docking identified core components, including furanogermenone and curcumenol, predicted to exert anti\u2010insomnia effects by targeting ADORA2A, GABRB3, and SLC6A4 and regulating the neuroactive ligand\u2010receptor interaction pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study characterizes the in\u00a0vivo migration characteristics of JYAS\u2010G and provides insights into its multi\u2010target anti\u2010insomnia mechanism, while comparing the modeling differences between two common insomnia animal models. These findings provide scientific evidence for the rational clinical application and further development of JYAS\u2010G.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.70195","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T07:34:05Z","timestamp":1791876845000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Migration Characteristics and Mechanism of Active Components From Jieyu Anshen Granule in Different Insomnia Mouse Models"],"prefix":"10.1002","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2361-1704","authenticated-orcid":false,"given":"Shiqi","family":"Yan","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengting","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Postgraduate Union Training Base of Xiangyang No. 1 People's Hospital, School of Medicine Wuhan University of Science and Technology  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Run","family":"Wan","sequence":"additional","affiliation":[{"name":"Department of Rehabilitation Medicine, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Ge","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Xiangyang Central Hospital, The Affiliated Hospital of Hubei University of Arts and Science  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mo","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Georgia Institute of Technology  Atlanta Georgia USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Hubei Provincial Clinical Research Center for Parkinson's Disease, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoqun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, Xiangyang No. 1 People's Hospital Hubei University of Medicine  Xiangyang Hubei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3649-2428","authenticated-orcid":false,"given":"Chenning","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of GCP Office, Taihe Hospital Hubei University of Medicine  Shiyan Hubei China"},{"name":"National Key Laboratory on Technologies for Chinese Medicine Pharmaceutical Process Control and Intelligent Manufacture Jiangsu Kanion Pharmaceutical Co. 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