{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:19:34Z","timestamp":1761513574545,"version":"build-2065373602"},"reference-count":54,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T00:00:00Z","timestamp":1421280000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2015,2,28]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Mass spectrometry imaging (MSI) can provide accurate data containing the spatial distribution of endogenous peptides in tissue sections without previous treatment. One of the key issues in analyzing small samples is establishing a proper technique for mounting and manipulating collected tissue in order to avoid contamination of the sample with optimal cutting temperature (OCT) resin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We present a method for embedding rat pituitary tissue in a frozen egg yolk block, which enables its further imaging in experiments on a matrix\u2010assisted laser desorption\/ionization (MALDI) mass spectrometer with time\u2010of\u2010flight (TOF) analyzer. Embedding the sample in the egg yolk prevents contamination from the OCT resin, which decreases MALDI signal quality.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In the present study we detected numerous\n                      <jats:italic>m\/z<\/jats:italic>\n                      peaks related to endogenous peptides. We identified fifteen peptides and their post\u2010translational modifications by tandem mass spectrometry (MS\/MS) directly on tissue sections of the hypophysis posterior and intermediate lobes; among these peptides were vasopressin, oxytocin, copeptin, melanocyte\u2010stimulating hormones and beta\u2010endorphin. We also showed that egg yolk itself does not affect localization of peptides in the pituitary.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Egg yolk embedding enables preparation of tissue sections from small tissue fragments to organs such as the pituitary gland, which is suitable for localization and identification of endogenous peptides by the MALDI\u2010MSI and MALDI\u2010MS\/MS techniques. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7112","type":"journal-article","created":{"date-parts":[[2015,1,15]],"date-time":"2015-01-15T06:57:29Z","timestamp":1421305049000},"page":"327-335","source":"Crossref","is-referenced-by-count":5,"title":["Imaging and identification of endogenous peptides from rat pituitary embedded in egg yolk"],"prefix":"10.1002","volume":"29","author":[{"given":"Piotr","family":"Sosnowski","sequence":"first","affiliation":[{"name":"Faculty of Chemistry, Biological and Chemical Research Centre University of Warsaw  Pasteura 1 02\u2010093 Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tymoteusz","family":"Zera","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Physiology Laboratory of Centre for Preclinical Research, The Medical University of Warsaw  Banacha 1B 02\u2010097 Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beata","family":"Wilenska","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biological and Chemical Research Centre University of Warsaw  Pasteura 1 02\u2010093 Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ewa","family":"Szczepanska\u2010Sadowska","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Physiology Laboratory of Centre for Preclinical Research, The Medical University of Warsaw  Banacha 1B 02\u2010097 Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Misicka","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, Biological and Chemical Research Centre University of Warsaw  Pasteura 1 02\u2010093 Warsaw Poland"},{"name":"Mossakowski Medical Research Centre Polish Academy of Sciences  Pawinskiego 5 02\u2010106 Warsaw Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,1,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac990781q"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2013.03.006"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2012.03.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.03.032"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2012.04.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1586\/14789450.2013.814939"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr400743a"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coph.2013.06.003"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2013.01.018"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2013.07.033"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2013.06.015"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erl139"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290020802"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200701121"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.R800051-JLR200"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11481-009-9162-6"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwp089"},{"key":"e_1_2_6_19_1","first-page":"e3445","volume":"60","author":"Hanrieder J.","year":"2012","journal-title":"J. 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