{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:05:45Z","timestamp":1791201945660,"version":"4.2.0"},"reference-count":82,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2015,5,28]],"date-time":"2015-05-28T00:00:00Z","timestamp":1432771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2015,7,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      The bias associated with lipid contents in fish tissues is a recalcitrant topic for trophic studies using stable isotopes. Lipids are depleted in the heavy carbon isotope (\n                      <jats:sup>13<\/jats:sup>\n                      C) and the lipid content varies considerably among species, tissues and in both time and space. We have applied and assessed different correction methods for tropical tuna tissues.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We tested two types of normalization methods to deal with variable lipid content in liver, gonads, and white and red muscles of yellowfin, bigeye and skipjack tuna: a chemical extraction using dichloromethane and a mathematical correction based on three modeling approaches (linear, non\u2010linear and mass balance models). We measured isotopic ratios of bulk and lipid\u2010free tissues and assessed the predictive ability of the correction models with the lipid\u2010free measurements. The parameters of the models were estimated from our dataset and from results from published studies on other species.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparison between bulk, lipid\u2010free and lipid\u2010corrected isotopic ratios demonstrated that (1) chemical extraction using dichloromethane did not affect \u03b4\n                      <jats:sup>15<\/jats:sup>\n                      N values; (2) the change in \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C values after extraction was tissue\u2010specific; (3) lipid\u2010normalization models using published parameter estimates failed to predict lipid\u2010corrected \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C values; and (4) linear and non\u2010linear models using parameters estimated for each tissue from our dataset provided accurate \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C predictions for all tissues, and mass balance model for white muscle only.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Models using published estimates for parameters from other species cannot be used. Based on a range of lipid content that do not exceed 45%, we recommend the linear model to correct the bulk \u03b4\n                      <jats:sup>13<\/jats:sup>\n                      C values in the investigated tissues but the parameters have to be estimated from a proportion of the original data for which chemical extraction is required and the isotopic values of bulk and lipid\u2010free tissues are measured. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7215","type":"journal-article","created":{"date-parts":[[2015,10,13]],"date-time":"2015-10-13T17:28:44Z","timestamp":1444757324000},"page":"1253-1267","source":"Crossref","is-referenced-by-count":26,"title":["Methods of lipid\u2010normalization for multi\u2010tissue stable isotope analyses in tropical tuna"],"prefix":"10.1002","volume":"29","author":[{"given":"Fany","family":"Sardenne","sequence":"first","affiliation":[{"name":"IRD, UMR MARine Biodiversity Exploitation and Conservation (MARBEC)  Avenue Jean Monnet S\u00e8te France"},{"name":"IRD, UMR MARine Biodiversity Exploitation and Conservation (MARBEC)  Fishing Port Victoria Seychelles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"M\u00e9nard","sequence":"additional","affiliation":[{"name":"IRD, Mediterranean Institute of Oceanography (MIO), Aix\u2010Marseille Universit\u00e9\/CNRS\/IRD\/Universit\u00e9 de Toulon  13288 Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maxime","family":"Degroote","sequence":"additional","affiliation":[{"name":"IRD, UMR MARine Biodiversity Exploitation and Conservation (MARBEC)  Avenue Jean Monnet S\u00e8te France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edwin","family":"Fouch\u00e9","sequence":"additional","affiliation":[{"name":"IRD, UMR MARine Biodiversity Exploitation and Conservation (MARBEC)  Avenue Jean Monnet S\u00e8te France"},{"name":"INRA, UMR TOXALIM (Research Centre in Food Toxicology)  Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ga\u00ebl","family":"Guillou","sequence":"additional","affiliation":[{"name":"CNRS\u2010Universit\u00e9 de la Rochelle, UMR Littoral Environnement et Soci\u00e9t\u00e9s (LIENSs)  2 rue Olympe de Gouges 17000 La Rochelle France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Lebreton","sequence":"additional","affiliation":[{"name":"CNRS\u2010Universit\u00e9 de la Rochelle, UMR Littoral Environnement et Soci\u00e9t\u00e9s (LIENSs)  2 rue Olympe de Gouges 17000 La Rochelle France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie J.","family":"Hollanda","sequence":"additional","affiliation":[{"name":"Seychelles Fishing Authority (SFA)  Fishing Port Victoria Seychelles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Bodin","sequence":"additional","affiliation":[{"name":"IRD, UMR MARine Biodiversity Exploitation and Conservation (MARBEC)  Fishing Port Victoria Seychelles"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,5,28]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"138","volume-title":"Stable Isotopes in Ecology and Environmental Science","author":"Michener R. 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