{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T01:23:05Z","timestamp":1769217785296,"version":"3.49.0"},"reference-count":25,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2002,6,20]],"date-time":"2002-06-20T00:00:00Z","timestamp":1024531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2002,7,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    An original and highly specific method able to identify and quantify traces of five tetracycline antibiotics (TCAs) in milk and eggs is presented. This method uses a single solid\u2010phase extraction (SPE) cartridge for simultaneous extraction and purification of TCAs in the above matrices. After diluting 5\u2005mL of intact whole milk or 2\u2005g egg samples with Na\n                    <jats:sub>2<\/jats:sub>\n                    EDTA\u2010containing water, samples are passed through a 0.5\u2010g Carbograph 4 extraction cartridge. After analyte elution from the SPE cartridge, an aliquot of the final extract is injected into a liquid chromatography\/mass spectrometry (LC\/MS) instrument equipped with an electrospray ion source and a single quadrupole. MS data acquisition is performed in the positive\u2010ion mode and by a time\u2010scheduled multiple\u2010ion selected ion\u2010monitoring program. With methanol as organic modifier, the in\u2010source collision\u2010induced dissociation (CID) process generated fragment ions able to pick up one methanol molecule. In several cases, these methanol\u2010adduct fragment ions have\n                    <jats:italic>m\/z<\/jats:italic>\n                    values higher than those of the protonated molecules. This event is rarely encountered in MS, thus making the analysis of TCAs by this method extremely specific. Compared with a conventional published method, the present protocol extracted larger amounts of TCAs from both milk and egg and decreased the analysis time by a factor of 3. Recovery of TCAs in milk at the 25\u2010ppb level ranged between 81 and 96% with relative standard deviation (RSD) no larger than 9%. Recovery of TCAs in egg at the 50\u2010ppb level ranged between 72 and 92% with RSD no larger than 7%. Estimated limits of quantification(S\/N\u2005=\u200510) of the method were 2\u20139 ppb TCAs in whole milk and 2\u201319 ppb TCAs in eggs. Copyright\u00a9 2002 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.724","type":"journal-article","created":{"date-parts":[[2002,10,6]],"date-time":"2002-10-06T07:34:39Z","timestamp":1033889679000},"page":"1365-1376","source":"Crossref","is-referenced-by-count":45,"title":["An original approach to determining traces of tetracycline antibiotics in milk and eggs by solid\u2010phase extraction and liquid chromatography\/mass spectrometry"],"prefix":"10.1002","volume":"16","author":[{"given":"Federica","family":"Bruno","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roberta","family":"Curini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Di","family":"Corcia","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Nazzari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"Pallagrosi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2002,6,20]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/02652038909373740"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/02652039109373956"},{"key":"e_1_2_6_4_2","unstructured":"CarsonMC RighterRF WagnerDD. InResidues of veterinary drugs and mycotoxins in animal products. Proc. 1st Electronic Mail Conference EnneG KuiperHA ValentiniA(eds). 15 April\u201331 August 1994 Wageningen Netherlands Wageningen Press 1994 76\u201380."},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf000144x"},{"key":"e_1_2_6_6_2","first-page":"51","volume":"92","author":"Wegener HC","year":"1999","journal-title":"Acta Vet. Scand. Suppl."},{"key":"e_1_2_6_7_2","unstructured":"Council Regulation(EC) No. 2377\/90 as modified by the Council Regulation No. 281\/96 laying down a Community procedure for the establishment of maximum residue limits of veterinary medicinal products in foodstuffs of animal origin. Official Journal of the EC No L37\/9 15\/2\/1996."},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/a804913k"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00280-5"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00477-6"},{"key":"e_1_2_6_11_2","first-page":"77","volume-title":"Chemical Analysis for Antibiotics Used in Agriculture","author":"Boison JO","year":"1995"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)01316-3"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00048-X"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(98)00119-8"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf010046r"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0021-9673(95)00953-1"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00260-1"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9603160"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19990915)13:17<1744::AID-RCM709>3.0.CO;2-I"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(96)00524-5"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(97)01278-8"},{"key":"e_1_2_6_22_2","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1002\/(SICI)1097-0231(20000229)14:4<280::AID-RCM880>3.0.CO;2-J","volume":"14","author":"Van Eeckout N","year":"2000","journal-title":"Rapid Commun. 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