{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:01Z","timestamp":1786978381223,"version":"build-2736575974"},"reference-count":31,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2015,8,6]],"date-time":"2015-08-06T00:00:00Z","timestamp":1438819200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2015,9,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      An isotopic labeling strategy based on derivatizing amine\u2010containing metabolites has been developed using light (\n                      <jats:sup>12<\/jats:sup>\n                      C\n                      <jats:sub>6<\/jats:sub>\n                      ) and heavy (\n                      <jats:sup>13<\/jats:sup>\n                      C\n                      <jats:sub>6<\/jats:sub>\n                      )\n                      <jats:italic>N<\/jats:italic>\n                      \u2010benzoyloxysuccinimide reagents for semi\u2010targeted metabolomic applications.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Differentially labeled samples were combined and analyzed simultaneously by liquid chromatography\/high\u2010resolution tandem mass spectrometry (LC\/HR\u2010MS\/MS) to compare relative amounts of amine\u2010containing metabolites. The selectivity of the reaction was determined with model metabolites and was shown to also be applicable to thiol and phenol moieties. The potential for relative quantitation was evaluated in cell extracts and the method was then applied to quantify metabolic perturbations occurring in human cultured cells under normal\n                      <jats:italic>vs.<\/jats:italic>\n                      oxidative stress conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 279 derivatized features were detected in HL60 cell extracts, 77 of which yielded significant concentration changes upon oxidative stress treatment. Based on accurate mass measurements and MS\/MS spectral matching with reference standard solutions, 10 metabolites were clearly identified. Derivatized compounds were found to have diagnostic fragment ions from the reagent itself, as well as structurally informative ions useful for metabolite identification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This simple derivatization reaction can be applied to the relative quantitation of amine\u2010, thiol\u2010 and phenol\u2010containing compounds, with improved sensitivity and chromatographic peak shapes due to the increased hydrophobicity of polar metabolites not readily amenable to reversed\u2010phase LC\/MS analysis. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7264","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:22:11Z","timestamp":1439241731000},"page":"1632-1640","source":"Crossref","is-referenced-by-count":14,"title":["Isotope\u2010labeled differential profiling of metabolites using\n                    <i>N<\/i>\n                    \u2010benzoyloxysuccinimide derivatization coupled to liquid chromatography\/high\u2010resolution tandem mass spectrometry"],"prefix":"10.1002","volume":"29","author":[{"given":"Michel","family":"Wagner","sequence":"first","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al Chemistry Department\/Pharmaqam  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leanne B.","family":"Ohlund","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al Chemistry Department\/Pharmaqam  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tze Chieh","family":"Shiao","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al Chemistry Department\/Pharmaqam  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Am\u00e9lie","family":"V\u00e9zina","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al Chemistry Department\/Pharmaqam  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Borhane","family":"Annabi","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al Chemistry Department\/Pharmaqam  Montr\u00e9al QC Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ren\u00e9","family":"Roy","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Montr\u00e9al 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