{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T06:08:44Z","timestamp":1773295724789,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2015,10,28]],"date-time":"2015-10-28T00:00:00Z","timestamp":1445990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2015,12,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Compound\u2010specific isotope analysis (CSIA) of nitrogen in amino acids has proven a valuable tool in many fields (e.g. ecology). Several intact polar lipids (IPLs) also contain nitrogen, and their nitrogen isotope ratios have the potential to elucidate food\u2010web interactions or metabolic pathways. Here we have developed novel methodology for the determination of \u03b4\n                      <jats:sup>15<\/jats:sup>\n                      N values of nitrogen\u2010containing headgroups of IPLs using gas chromatography coupled with isotope\u2010ratio mass spectrometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Intact polar lipids with nitrogen\u2010containing headgroups were hydrolyzed and the resulting compounds were derivatized by (1) acetylation with pivaloyl chloride for compounds with amine and hydroxyl groups or (2) esterification using acidified 2\u2010propanol followed by acetylation with pivaloyl chloride for compounds with both carboxyl and amine groups. The \u03b4\n                      <jats:sup>15<\/jats:sup>\n                      N values of the derivatives were subsequently determined using gas chromatography\/combustion\/isotope\u2010ratio mass spectrometry.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Intact polar lipids with ethanolamine and amino acid headgroups, such as phosphatidylethanolamine and phosphatidylserine, were successfully released from the IPLs and derivatized. Using commercially available pure compounds it was established that \u03b4\n                      <jats:sup>15<\/jats:sup>\n                      N values of ethanolamine and glycine were not statistically different from the offline\u2010determined values. Application of the technique to microbial cultures and a microbial mat showed that the method works well for the release and derivatization of the headgroup of phosphatidylethanolamine, a common IPL in bacteria.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A method to enable CSIA of nitrogen of selected IPLs has been developed. The method is suitable for measuring natural stable nitrogen isotope ratios in microbial lipids, in particular phosphatidylethanolamine, and will be especially useful for tracing the fate of nitrogen in deliberate tracer experiments. Copyright \u00a9 2015 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7393","type":"journal-article","created":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T02:35:28Z","timestamp":1446431728000},"page":"2263-2271","source":"Crossref","is-referenced-by-count":5,"title":["Compound\u2010specific stable isotope analysis of nitrogen\u2010containing intact polar lipids"],"prefix":"10.1002","volume":"29","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5565-3266","authenticated-orcid":false,"given":"Elisabeth","family":"Svensson","sequence":"first","affiliation":[{"name":"Department of Marine Organic Biogeochemistry NIOZ Royal Netherlands Institute for Sea Research  P.O. Box\u00a059 1790 AB Den Burg (Texel) The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Schouten","sequence":"additional","affiliation":[{"name":"Department of Marine Organic Biogeochemistry NIOZ Royal Netherlands Institute for Sea Research  P.O. Box\u00a059 1790 AB Den Burg (Texel) The Netherlands"},{"name":"Department of Earth Sciences, Faculty of Geosciences Utrecht University  P.O. Box\u00a080021 3508 TA Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Axel","family":"Stam","sequence":"additional","affiliation":[{"name":"Department of Marine Organic Biogeochemistry NIOZ Royal Netherlands Institute for Sea Research  P.O. Box\u00a059 1790 AB Den Burg (Texel) The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack J.","family":"Middelburg","sequence":"additional","affiliation":[{"name":"Department of Earth Sciences, Faculty of Geosciences Utrecht University  P.O. Box\u00a080021 3508 TA Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaap S.","family":"Sinninghe Damst\u00e9","sequence":"additional","affiliation":[{"name":"Department of Marine Organic Biogeochemistry NIOZ Royal Netherlands Institute for Sea Research  P.O. Box\u00a059 1790 AB Den Burg (Texel) The Netherlands"},{"name":"Department of Earth Sciences, Faculty of Geosciences Utrecht University  P.O. Box\u00a080021 3508 TA Utrecht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,28]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.4319\/lo.2007.52.2.0780"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2009.138"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/gbi.12017"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6941.2002.tb00940.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-03377-7"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-7037(98)00076-3"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.quascirev.2011.01.012"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0903030106"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.orggeochem.2007.11.001"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-7037(02)00981-X"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ngeo983"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4707"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2009.01.013"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2011.60"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2005.3.230"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marchem.2012.12.001"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2007.5.192"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199604)31:4<367::AID-JMS310>3.0.CO;2-V"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.3354\/meps342085"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.4319\/lom.2009.7.740"},{"key":"e_1_2_6_22_1","unstructured":"DSMZ.Catalogue of Microorganisms. 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