{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T19:50:08Z","timestamp":1781812208061,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T00:00:00Z","timestamp":1453075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2014 M560949"],"award-info":[{"award-number":["2014 M560949"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["21475073"],"award-info":[{"award-number":["21475073"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007515","name":"National Youth Science Foundation","doi-asserted-by":"publisher","award":["21305074"],"award-info":[{"award-number":["21305074"]}],"id":[{"id":"10.13039\/100007515","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,2,28]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Breast cancer is the leading cause of cancer death among women worldwide. Identification of lipid targets that play a role in breast cancer invasion may advance our understanding of the rapid progression of cancer and may lead to the development of new biomarkers for the disease.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Matrix\u2010assisted laser desorption\/ionization mass spectrometry imaging (MALDI MSI) was applied for the lipidomic profiling of two poorly invasive and two highly invasive breast cancer cell lines to identify the differentially accumulated lipids related to the invasive phenotype. The four cell lines were individually grown on indium tin oxide (ITO)\u2010coated glass slides, analyzed as cell cultures. The raster width and matrix for detection were optimized to improve detection sensitivity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Optimized MSI measurements were performed directly on the cell culture with 9\u2010aminoacridine as matrix, resulting in 215 endogenous compounds detected in positive ion mode and 267 endogenous compounds in negative ion mode in all the four cell lines, representing the largest group of analytes that have been analyzed from cells by a single MSI study. In highly invasive cell lines, 31 lipids including phosphatidylglycerol (PG) and phosphatidic acids were found upregulated and eight lipids including sphingomyelin (SM) downregulated in negative ion mode. The products of\n                      <jats:italic>de novo<\/jats:italic>\n                      fatty acid synthesis incorporated into membrane phospholipids, like oleic\u2010acid\u2010containing PG, may be involved in mitochondrial dysfunction and thus affect the invasion of breast cancer cells. The deficiency of SM may be related to the disruption of apoptosis in highly invasive cancer cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This work uncovered more analytes in cells by MSI than previous reports, providing a better visualization and novel insights to advance our understanding of the relationship between rapid progression of breast cancer and lipid metabolism. The most altered lipids may aid the discovery of diagnostic markers and therapeutic targets of breast cancer. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7466","type":"journal-article","created":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T01:13:09Z","timestamp":1453079589000},"page":"533-542","source":"Crossref","is-referenced-by-count":39,"title":["Matrix\u2010assisted laser desorption\/ionization mass spectrometry imaging of cell cultures for the lipidomic analysis of potential lipid markers in human breast cancer invasion"],"prefix":"10.1002","volume":"30","author":[{"given":"Shujuan","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaowu","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hemi","family":"Luan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhai","family":"Lin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongwei","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry Hong Kong Baptist University  Hong Kong SAR China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianjun","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Modern Toxicology of Shenzhen, Shenzhen Center for Disease Control and Prevention  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"Key Laboratory of Metabolomics at Shenzhen, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyang","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology, Graduate School at Shenzhen Tsinghua University  Shenzhen 518055 China"},{"name":"School of Medicine Tsinghua University  Beijing 10084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,1,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)61351-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20073"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molonc.2014.06.007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(10)70008-5"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27997"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M112.019786"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr4012156"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209265"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1776"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2934"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrn3012"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2172"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2222"},{"key":"e_1_2_6_15_1","first-page":"2115","article-title":"Enzymes of the fatty acid synthesis pathway are highly expressed in in situ breast carcinoma","volume":"3","author":"Milgraum L. 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