{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,25]],"date-time":"2026-01-25T14:51:31Z","timestamp":1769352691875,"version":"3.49.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,2,8]],"date-time":"2016-02-08T00:00:00Z","timestamp":1454889600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rational<\/jats:title>\n                    <jats:p>\n                      Analysis of bacteria by matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOFMS) often relies upon sample preparation methods that result in cell lysis, e.g. bead\u2010beating. However, Shiga toxin\u2010producing\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      (STEC) can undergo bacteriophage\u2010induced cell lysis triggered by antibiotic exposure that may allow greater selectivity of the proteins extracted.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We have developed a sample preparation method for selective extraction of bacteriophage\u2010encoded proteins and specifically Shiga toxins 1 and 2 (Stx1 &amp; 2) expressed from STEC strains induced by DNA\u2010damaging antibiotics. STEC strains were cultured overnight on agar supplemented with ciprofloxacin, mitomycin\u2010C or an iron chelator to induce the bacteriophage lytic cycle with concomitant expression and release of Stx1 and\/or Stx2. Sample preparation relied exclusively on bacteriophage lysis for release Stx into the extraction solution.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Three clinical STEC strains were analyzed by matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF\u2010TOF\u2010MS\/MS) and top\u2010down proteomics analysis:\n                      <jats:italic>E. coli<\/jats:italic>\n                      O157:H7 strain EDL933,\n                      <jats:italic>E. coli<\/jats:italic>\n                      O91:H21 strain B2F1 and\n                      <jats:italic>E. coli<\/jats:italic>\n                      O26:H11 strain ECRC #05.2217. The B\u2010subunit of Stx1a of EDL933 was detected and identified even though it was ~100\u2010fold less abundant than the B\u2010subunit of Stx2a that had been identified previously for this strain. Two bacteriophage\u2010encoded proteins were also identified: L0117 and L0136. The B\u2010subunits of Stx2d of strain B2F1 and Stx1a of strain ECRC #05.2217 were also detected and identified.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Bacteriophage lysis appeared to enhance the detection sensitivity of Stx for these STEC strains compared to previous work using mechanical lysis. Detection\/identification of other bacteriophage\u2010encoded proteins (beyond Stx) tends to support the hypothesis of Stx release by bacteriophage cell lysis. Published in 2016. This article is a U.S. Government work and is in the public domain in the USA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7507","type":"journal-article","created":{"date-parts":[[2016,2,9]],"date-time":"2016-02-09T06:50:04Z","timestamp":1455000604000},"page":"671-680","source":"Crossref","is-referenced-by-count":17,"title":["Bacteriophage cell lysis of Shiga toxin\u2010producing\n                    <i>Escherichia coli<\/i>\n                    for top\u2010down proteomic identification of Shiga toxins 1 &amp; 2 using matrix\u2010assisted laser desorption\/ionization tandem time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"30","author":[{"given":"Clifton K.","family":"Fagerquist","sequence":"first","affiliation":[{"name":"Produce Safety &amp; Microbiology Research Unit Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture  800 Buchanan Street Albany CA 94710 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William J.","family":"Zaragoza","sequence":"additional","affiliation":[{"name":"Produce Safety &amp; Microbiology Research Unit Western Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture  800 Buchanan Street Albany CA 94710 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290081224"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1196-1584"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960731)10:10<1227::AID-RCM659>3.0.CO;2-6"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0231(19960610)10:8<883::AID-RCM594>3.0.CO;2-V"},{"key":"e_1_2_6_6_1","unstructured":"http:\/\/www.fda.gov\/NewsEvents\/Newsroom\/PressAnnouncements\/ucm365907.htm."},{"key":"e_1_2_6_7_1","unstructured":"http:\/\/www.accessdata.fda.gov\/cdrh_docs\/reviews\/K130831.pdf."},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-014-2183-y"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01996-13"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00157-08"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2013.07.021"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac902455d"},{"key":"e_1_2_6_13_1","article-title":"Source\u2010identifying biomarker ions between environmental and clinical Burkholderia pseudomallei using whole\u2010cell matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry (MALDI\u2010TOF MS)","volume":"10","author":"Niyompanich S.","year":"2014","journal-title":"PLoS One"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac051419g"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.599"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1046-5928(02)00518-1"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/b000368i"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.10.6292-6307.2005"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac040193z"},{"key":"e_1_2_6_20_1","first-page":"173","article-title":"Increased protein yields from Escherichia coli using pressure\u2010cycling technology","volume":"17","author":"Smejkal G. 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