{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:29:12Z","timestamp":1761485352426,"version":"build-2065373602"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2016,4,5]],"date-time":"2016-04-05T00:00:00Z","timestamp":1459814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["CZ.1.05\/2.1.00\/01.0024","LO1205"],"award-info":[{"award-number":["CZ.1.05\/2.1.00\/01.0024","LO1205"]}]},{"name":"Grant Agency of the University of South Bohemia in Ceske Budejovice","award":["012\/2016\/Z","084\/2016\/Z"],"award-info":[{"award-number":["012\/2016\/Z","084\/2016\/Z"]}]},{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"crossref","award":["GACR P503\/11\/1130"],"award-info":[{"award-number":["GACR P503\/11\/1130"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,5,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Diltiazem, a calcium channel blocker drug, is widespread in the environment because of its incomplete elimination during water treatment. It can cause negative effects on aquatic organisms; thus, a rapid and sensitive liquid chromatography\/mass spectrometry (LC\/MS) method to detect its presence was developed. Our approach is based on accurate mass measurements using a hybrid quadrupole\u2010orbital trap mass spectrometer that was used to measure diltiazem and its metabolites in fish tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Blood plasma, muscle, liver, and kidney tissues of rainbow trout (\n                      <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                      ), exposed for 42 days to 30 \u03bcg L\n                      <jats:sup>\u20131<\/jats:sup>\n                      diltiazem, were used for the method development. No metabolite standards were required to identify the diltiazem biotransformation products in the fish tissue.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Overall, 17 phase I diltiazem metabolites (including isomeric forms) were detected and tentatively identified using the MassFrontier spectral interpretation software. A semi\u2010quantitative approach was used for organ\u2010dependent comparison of the metabolite concentrations.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data increase our understanding about diltiazem and its metabolites in aquatic organisms, such as fish. These encompass desmethylation, desacetylation and hydroxylation as well as their combinations. This study represents the first report of the complex diltiazem phase I metabolic pathways in fish. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7543","type":"journal-article","created":{"date-parts":[[2016,4,10]],"date-time":"2016-04-10T20:55:29Z","timestamp":1460321729000},"page":"1153-1162","source":"Crossref","is-referenced-by-count":2,"title":["<b>Investigation of diltiazem metabolism in fish using a hybrid quadrupole\/orbital trap mass spectrometer<\/b>"],"prefix":"10.1002","volume":"30","author":[{"given":"Olga","family":"Koba","sequence":"first","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Steinbach","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana Kocour","family":"Kroupov\u00e1","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate\u0159ina","family":"Grabicov\u00e1","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Rand\u00e1k","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roman","family":"Grabic","sequence":"additional","affiliation":[{"name":"University of South Bohemia in Ceske Budejovice Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and Hydrobiology  Zatisi 728\/II 389 25 Vodnany Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,4,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986707779941122"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac800066n"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncl.2008.11.007"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2003.07.001"},{"issue":"36","key":"e_1_2_6_6_1","first-page":"1202","article-title":"Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999\u20132000: A national reconnaissance","volume":"2002","author":"Kolpin D. 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