{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,4]],"date-time":"2026-04-04T07:15:30Z","timestamp":1775286930458,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,5,15]],"date-time":"2016-05-15T00:00:00Z","timestamp":1463270400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,6,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Investigation of nanoparticles for laser desorption\/ionization mass spectrometry (LDI\u2010MS) is routinely reported. However, the effect of surface capping of nanomaterials for LDI\u2010MS is not well studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Different capping agents of quantum dots (CdTe) affect the spectra quality and sensitivity of protein analysis and protein digestion using trypsin enzyme assisted by microwave. Surface modification of CdTe quantum dots with different capping agents, namely 3\u2010mercaptopropionic acid (3\u2010MPA), 4\u2010aminothiophenol (4\u2010ATP), 4\u2010mercaptobenzoic acid (4\u2010MBA), 11\u2010mercaptoundecanoic acid (11\u2010MUA), cysteine (Cys) and thioglycolic acid (TG), were investigated for quantum dots (QDs)\u2010assisted trypsin protease followed by analysis using mass spectrometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CdTe QDs were used as a surface to assist trypsin protease and laser desorption\/ionization mass spectrometry (surface\u2010assisted laser desorption\/ionization mass spectrometry, SALDI\u2010MS). The MS profiles for the investigated analytes (bovine serum albumin (BSA), lysozyme, cytochrome c, \u03b1\u2010casein, transferrin and myoglobin) revealed almost the absence of degradation that implies the softness of the present technique. QDs\u2010assisted LDI\u2010MS offered high sensitivity and high resolution. QDs showed significant enhancement of microwave\u2010assisted trypsin digestion of the investigated proteins and these improvements boosted the identifications of fragments with a database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A capping agent of quantum dots affects the analysis of proteins and peptides using LDI\u2010MS. CdTe QDs offer sensitive, high\u2010resolution and simple analysis of proteins. QDs improved the protein digestion using the microwave\u2010assisted trypsin digestion. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7575","type":"journal-article","created":{"date-parts":[[2016,5,22]],"date-time":"2016-05-22T12:10:21Z","timestamp":1463919021000},"page":"1403-1412","source":"Crossref","is-referenced-by-count":38,"title":["<b>Effect of surface capping of quantum dots (CdTe) on proteomics<\/b>"],"prefix":"10.1002","volume":"30","author":[{"given":"Zhen\u2010Yu","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Chemistry and Center for Nanoscience and Nanotechnology National Sun Yat\u2010Sen University  Kaohsiung 80424 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hani Nasser","family":"Abdelhamid","sequence":"additional","affiliation":[{"name":"Department of Chemistry Assuit University  Assuit 71515 Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Fen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Center for Nanoscience and Nanotechnology National Sun Yat\u2010Sen University  Kaohsiung 80424 Taiwan"},{"name":"School of Pharmacy, College of Pharmacy Kaohsiung Medical University  Kaohsiung 807 Taiwan"},{"name":"Institute of Medical Science and Technology National Sun Yat\u2010Sen University  Kaohsiung 80424 Taiwan"},{"name":"Doctoral Degree Program in Marine Biotechnology National Sun Yat\u2010Sen University and Academia Sinica  Kaohsiung 80424 Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,5,15]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmat3984"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/jacs.5b02026"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nphoton.2015.36"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/lsa.2015.7"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3tb21020k"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5RA07069D"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA07638A"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201200295"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa2501"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/adma.201405222"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nnano.2014.298"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290020802"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C0CS00050G"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.4028\/www.scientific.net\/MSF.832.28"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2013.11.078"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4RA07250B"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN02158D"},{"key":"e_1_2_6_19_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.colsurfb.2013.11.013","article-title":"Polymer dots for quantifying the total hydrophobic pathogenic lysates in a single drop","volume":"115","author":"Abdelhamid H. 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