{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:04:54Z","timestamp":1790485494388,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2016,6,22]],"date-time":"2016-06-22T00:00:00Z","timestamp":1466553600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,7,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Electrospray ionization mass spectrometry (ESI\u2010MS)\u2010based techniques commonly used in oligonucleotide analyses are known to be sensitive to alkali metal adduct formation. Adducts directly impact the sensitivity of MS\u2010based analyses as the available charge is distributed across the parent peak and adduct(s). The current study systematically evaluated common liquid chromatography (LC) components in LC\/ESI\u2010MS configurations used in oligonucleotide analysis to identify metal adduct contributions from LC instrumentation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A UPLC liquid chromatography system was configured with a single quadrupole MS detector (ACQUITY QDa, Waters Corp.) to monitor adduct formation in oligonucleotide separations. An ion\u2010pairing mobile phase comprised of 15\u00a0mM triethylamine and 400\u00a0mM hexafluoro\u20102\u2010propanol was used in conjunction with an oligonucleotide separation column (Waters OST BEH C18, 2.1\u00a0mm\u00a0\u00d7\u00a050\u00a0mm) for all separations. A 10\u2010min method was used to provide statistical figures of merit and evaluate adduct formation over time.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Trace alkali metal salts in the mobile phase and reagents were determined to be the main source of metal salt adducts in LC\/ESI\u2010MS\u2010based configurations. Non\u2010specific adsorption sites located throughout the fluidic path contribute to adduct formation in oligonucleotide analyses. Ion\u2010pairing mobile phases prepared at neutral or slightly basic pH result in up to a 57% loss of spectral abundance to adduct formation in the current study.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Implementation of a short low pH reconditioning step was observed to effectively displace trace metal salts non\u2010specifically adsorbed to surfaces in the fluidic path and was able to maintain an average MS spectral abundance \u226594% with a high degree of repeatability (relative standard deviation (R.S.D.) 0.8%) over an extended time study. The proposed method offers the ability to rapidly regenerate adsorption sites with minimal impact on productivity while retaining assay sensitivity afforded by MS detection with reduced adduct formation. \u00a9 2016 The Authors.\n                      <jats:italic>Rapid Communications in Mass Spectrometry<\/jats:italic>\n                      Published by John Wiley &amp; Sons Ltd.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7596","type":"journal-article","created":{"date-parts":[[2017,3,23]],"date-time":"2017-03-23T05:58:53Z","timestamp":1490248733000},"page":"1667-1679","source":"Crossref","is-referenced-by-count":49,"title":["Reduction of metal adducts in oligonucleotide mass spectra in ion\u2010pair reversed\u2010phase chromatography\/mass spectrometry analysis"],"prefix":"10.1002","volume":"30","author":[{"given":"Robert E.","family":"Birdsall","sequence":"first","affiliation":[{"name":"Waters Corp.  34 Maple St Milford MA 01757\u20103604 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Gilar","sequence":"additional","affiliation":[{"name":"Waters Corp.  34 Maple St Milford MA 01757\u20103604 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Shion","sequence":"additional","affiliation":[{"name":"Waters Corp.  34 Maple St Milford MA 01757\u20103604 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Waters Corp.  34 Maple St Milford MA 01757\u20103604 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weibin","family":"Chen","sequence":"additional","affiliation":[{"name":"Waters Corp.  34 Maple St Milford MA 01757\u20103604 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,6,22]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2011.09.007"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1367-5931(98)80129-4"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-7799(96)10053-6"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2006.03.015"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.535"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-199957030-00010"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa042760"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(91)90480-Y"},{"key":"e_1_2_5_10_1","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1182\/blood.V90.1.331.331_331_339","article-title":"Antisense oligodeoxyribonucleotides suppress hematologic cell growth through stepwise release of deoxyribonucleotides","volume":"90","author":"Vaerman J. 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