{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:53:01Z","timestamp":1786978381200,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T00:00:00Z","timestamp":1471478400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"crossref","award":["2014\u00a0A030313757"],"award-info":[{"award-number":["2014\u00a0A030313757"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007515","name":"National Youth Science Foundation","doi-asserted-by":"crossref","award":["21305074 and 21475073"],"award-info":[{"award-number":["21305074 and 21475073"]}],"id":[{"id":"10.13039\/100007515","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Single\u2010cell analysis is very important in several research fields for the heterogeneity of individual cells, which has been well accepted. However, restricted by the size and low content of a single cell, current studies have encountered challenges in high\u2010throughput, high\u2010space resolution and sensitivity, and multicomponent analysis. A methodology of a surface\u2010printed microdot array chip coupled with matrix\u2010assisted laser desorption ionization mass spectrometry (MALDI\u2010MS) is presented in this study for high\u2010throughput single\u2010cell patterning and phospholipid analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The poly\u2010L\u2010lysine (PLL) used as ink molecule was printed on an oxygen plasma processed indium tin oxide (ITO)\u2010coated glass slide to form a microdot array by micro\u2010contact printing technology. The cell array was then formed on the PLL microarray through electrostatic adsorption force. 9\u2010Aminoacridine (9\u2010AA) matrix was applied on the cell array before it was analyzed by MALDI\u2010TOF MS. MALDI mass spectrometry imaging (MALDI\u2010MSI) was then used for high\u2010throughput, quick measurement, and multicomponent analysis of the cell array.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The single\u2010cell capture efficiency of the cell array formed on the PLL microarray was about 40%. Twelve phospholipids were detected at the single\u2010cell level, and the structures were further confirmed by MS\/MS. The MALDI\u2010MSI of selected ions showed a conformity with the cell array. The relative signal intensity data of selected ions were extracted from every pixel in the image within several minutes. The heterogeneity between individual cells was revealed from the relative signal intensity of phospholipids in 1\u20133 cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Compared to the existing related approaches, high\u2010throughput, quick measurement, and multicomponent single\u2010cell analysis have been realized by our method. Through different ink molecules used for micro\u2010contact printing, the established platform could have the potential to capture and analyze specific cells. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7628","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T23:34:53Z","timestamp":1471563293000},"page":"73-79","source":"Crossref","is-referenced-by-count":20,"title":["Surface\u2010printed microdot array chips coupled with matrix\u2010assisted laser desorption\/ionization mass spectrometry for high\u2010throughput single\u2010cell patterning and phospholipid analysis"],"prefix":"10.1002","volume":"30","author":[{"given":"Ti","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Chemistry Tsinghua University  Beijing 100084 China"},{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology Graduate School at Shenzhen, Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology Graduate School at Shenzhen, Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jin","sequence":"additional","affiliation":[{"name":"Neptunus Pharmaceutical Technology Center  Shenzhen 518057 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuyang","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base\u2010Shenzhen Key Laboratory of Chemical Biology Graduate School at Shenzhen, Tsinghua University  Shenzhen 518055 China"},{"name":"School of Medicine Tsinghua University  Beijing 100084 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongxia","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Metabolomics at Shenzhen Graduate School at Shenzhen, Tsinghua University  Shenzhen 518055 China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pd.109"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00355"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5LC00652J"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9008463"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06965"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1423682112"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2006.05249.x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1072241"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-bioeng-070909-105238"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1019446108"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4LC01253D"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1lc20181f"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac2033084"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.276.5317.1425"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2lc40216e"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2008.08.017"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10544-013-9749-9"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2014.08.043"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0904764106"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6641"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/s150408981"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac2003592"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac202447t"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-6426-4"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac101565f"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(05)05001-9"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac200771g"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1360\/N032014-00007"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-015-8741-z"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1209302110"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)64849-5"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3LC51169C"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b00010"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1360\/N032013-00076"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac101998z"}],"container-title":["Rapid 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