{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T18:33:48Z","timestamp":1791657228215,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T00:00:00Z","timestamp":1471478400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Quantitative analysis of cholesterol and its metabolic steroid hormones plays a vital role in diagnosing endocrine disorders and understanding disease progression, as well as in clinical medicine studies. Because of their extremely low abundance in body fluids, it remains a challenging task to develop a sensitive detection method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A hyphenated technique of dual ultrasonic\u2010assisted dispersive liquid\u2013liquid microextraction (dual\u2010UADLLME) coupled with microwave\u2010assisted derivatization (MAD) was proposed for cleansing, enrichment and sensitivity enhancement. 4\u2032\u2010Carboxy\u2010substituted rosamine (CSR) was synthesized and used as derivatization reagent. An ultra\u2010high performance liquid chromatography tandem mass spectrometry (UHPLC\u2013MS\/MS) method was developed for determination of cholesterol and its metabolic steroid hormones in the multiple reaction monitoring mode.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Parameters of dual\u2010UADLLME, MAD and UHPLC\u2013MS\/MS were all optimized. Satisfactory linearity, recovery, repeatability, accuracy and precision, absence of matrix effect and extremely low limits of detection (LODs, 0.08\u20130.15\u00a0pg\u00a0mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      ) were achieved.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Through the combination of dual\u2010UADLLME and MAD, a determination method for cholesterol and its metabolic steroid hormones in human plasma, serum and urine samples was developed and validated with high sensitivity, selectivity, accuracy and perfect matrix effect results. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7634","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T23:36:12Z","timestamp":1471563372000},"page":"147-154","source":"Crossref","is-referenced-by-count":26,"title":["Sensitive determination of cholesterol and its metabolic steroid hormones by UHPLC\u2013MS\/MS via derivatization coupled with dual ultrasonic\u2010assisted dispersive liquid\u2013liquid microextraction"],"prefix":"10.1002","volume":"30","author":[{"given":"Xian\u2010En","family":"Zhao","sequence":"first","affiliation":[{"name":"Shandong Provincial Key Laboratory of Life\u2010Organic Analysis and Key Laboratory of Pharmaceutical Intermediates and Analysis of Natural Medicine, College of Chemistry and Chemical Engineering Qufu Normal University  Qufu 273165 Shandong China"},{"name":"Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry 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