{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T21:24:26Z","timestamp":1761513866075,"version":"build-2065373602"},"reference-count":24,"publisher":"Wiley","issue":"S1","license":[{"start":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T00:00:00Z","timestamp":1471478400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"MOST China","award":["2011YQ0900502"],"award-info":[{"award-number":["2011YQ0900502"]}]},{"name":"NNSF China","award":["81471919","212050059"],"award-info":[{"award-number":["81471919","212050059"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Antibiotic resistance in pathogenic bacteria is becoming a global public health problem, such as aminoglycoside resistance encoded by the\n                      <jats:italic>armA<\/jats:italic>\n                      gene. Although many methods have been reported, rapid analysis of environmental samples is still challenging.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>A rapid analytical method was developed in this study to determine bacterial aminoglycoside resistance using membrane electrospray ionization mass spectrometry (MESI\u2010MS). Precursor\/product\u2010ion pairs of ArmA unique peptides were detected with minimal sample preparation. Standard peptides were synthesized and used for developing and validating the methodology, and then the method was verified by both ArmA positive and ArmA negative simulated environmental samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A rapid method for determination of bacterial aminoglycoside resistance was developed using MESI\u2010MS\/MS. The bacterial cultural time was optimized to 2\u00a0hours, and the precision, accuracy and recovery of this method were investigated. The peptide IHSSTNER (IR\u20108) unique to ArmA in simulated environmental samples can be successfully identified within 3\u00a0hours.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The novel assay offered a rapid method to determine bacterial aminoglycoside resistance with high sensitivity, accuracy and precision in simulated environmental samples. This method could also be applied to identify other drug\u2010resistance proteins in clinical\/environmental samples. Copyright \u00a9 2016 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7648","type":"journal-article","created":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T23:35:10Z","timestamp":1471563310000},"page":"202-207","source":"Crossref","is-referenced-by-count":4,"title":["Rapid determination of bacterial aminoglycoside resistance in environmental samples using membrane electrospray ionization mass spectrometry"],"prefix":"10.1002","volume":"30","author":[{"given":"Liusheng","family":"Fan","sequence":"first","affiliation":[{"name":"School of Life Science Beijing Institute of Technology  Beijing 100081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming","family":"Ke","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing Institute of Technology  Beijing 100081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Yuan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ji","family":"Pu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinxing","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguo","family":"Xu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention  Beijing 102206 China"},{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases  Hangzhou 310003 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Xu","sequence":"additional","affiliation":[{"name":"School of Life Science Beijing Institute of Technology  Beijing 100081 China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,8,18]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2011.03570.x"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00058-07"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1145"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.124292"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00774-09"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2015.08.017"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0141315"},{"key":"e_1_2_6_9_1","article-title":"Emergence of plasmid\u2010mediated colistin resistance mechanism MCR\u20101 in animals and human beings in China: a microbiological and molecular biological study","author":"Liu Y.\u2010Y.","year":"2015","journal-title":"Lancet Infect. 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