{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T15:54:33Z","timestamp":1790438073218,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T00:00:00Z","timestamp":1469577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2016,8,30]]},"abstract":"\n Rationale<\/jats:title>\n \n The\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H values of some nitrogen\u2010containing organic compounds measured by High\u2010Temperature Conversion (HTC) with a glassy carbon reactor have been shown to be inaccurate. A probable explanation for these analytical inaccuracies is the formation of HCN, allowing some hydrogen atoms to escape isotope ratio measurement. We assess this isotopic effect in sample types commonly used for (paleo)ecological, environmental, archaeological, and forensic investigations.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n values and mass fraction H using a factory\u2010recommended glassy carbon HTC reactor packing were compared with those obtained from using two Cr\u2010containing reactor packings for a variety of N\u2010containing substances, including amino acids, collagen, hair, and silk.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n values and mass fraction H differed by reactor packing for most, but not all, N\u2010containing samples. The\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n difference was 10\u201311 \u2030 for modern collagen and 12\u201314 \u2030 for hair, demonstrating that reactor configuration is important for these proteins, and that the use of a chromium\u2010packed reactor may be desirable. In contrast,\n Bombyx mori<\/jats:italic>\n cocoon (silk)\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n values did not differ with reactor type. In general,\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n and mass fraction H differences by reactor packing increased with mass fraction nitrogen in the sample. With the Cr\u2010packed reactor hydrogen mass fractions were at theoretically expected values, while the glassy carbon reactor produced lower yields of hydrogen.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n The protein and amino acid\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H\n VSMOW\u2010SLAP<\/jats:sub>\n values measured by factory\u2010recommended online HTC methods differ from those from Cr\u2010containing reactor packing. The magnitude of the differences is variable with sample type; the molecular structure and diagenetic history of each sample may be important. Careful attention to this effect is therefore recommended for the\n \u03b4<\/jats:italic>\n 2<\/jats:sup>\n H measurement for all nitrogen\u2010containing analytes. Copyright \u00a9 2016 John Wiley & Sons, Ltd.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.7662","type":"journal-article","created":{"date-parts":[[2016,7,27]],"date-time":"2016-07-27T19:32:44Z","timestamp":1469647964000},"page":"1857-1864","source":"Crossref","is-referenced-by-count":17,"title":["Hydrogen isotopic analysis with a chromium\u2010packed reactor of organic compounds of relevance to ecological, archaeological, and forensic applications"],"prefix":"10.1002","volume":"30","author":[{"given":"Linda M.","family":"Reynard","sequence":"first","affiliation":[{"name":"Department of Human Evolutionary Biology Harvard University 11 Divinity Avenue Cambridge MA 02138 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noreen","family":"Tuross","sequence":"additional","affiliation":[{"name":"Department of Human Evolutionary Biology Harvard University 11 Divinity Avenue Cambridge MA 02138 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,27]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1","volume-title":"Handbook of Stable Isotope Analytical Techniques","author":"Groot P. 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