{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T19:41:11Z","timestamp":1790019671956,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006481","name":"Cotton Incorporated","doi-asserted-by":"publisher","award":["15\u2010851"],"award-info":[{"award-number":["15\u2010851"]}],"id":[{"id":"10.13039\/100006481","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2017,4,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Refined cottonseed oil has widespread applications in the food and chemical industries. Although the major lipids comprising cottonseed oil (triacylglycerols) are well known, there are many diverse lipid species in cotton seeds that occur at much lower levels and have important nutritional or anti\u2010nutritional properties.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The lipid technical samples were prepared in chloroform. The biological samples were extracted using a mixture of isopropanol\/chloroform\/H\n                      <jats:sub>2<\/jats:sub>\n                      O (2:1:0.45). The data were collected using high and low collision energy with simultaneous data collection on a time\u2010of\u2010flight (TOF) mass spectrometer which allowed the characterization of lipids by precursor and product ion alignment. The supercritical fluid chromatography methodology is flexible and can be altered to provide greater retention and separation. The comprehensive method was used to screen seed lipid extracts from several cotton genotypes using multivariate statistical analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Method variables influencing the peak integrity and chromatographic separation for a mixture of lipids with different degrees of polarity were explored. The experiments were designed to understand the chromatographic behavior of lipids in a controlled setting using a variety of lipid extracts. Influences of acyl chain length and numbers of double bonds were investigated using single moiety standards.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The methodology parameters were examined using single moiety lipid standards and standard mixtures. The method conditions were applied to biological lipid extracts, and adjustments were investigated to manipulate the chromatography. Insights from these method variable manipulations will help to frame the development of targeted lipid profiling and screening protocols. Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7825","type":"journal-article","created":{"date-parts":[[2017,1,10]],"date-time":"2017-01-10T10:37:02Z","timestamp":1484044622000},"page":"591-605","source":"Crossref","is-referenced-by-count":16,"title":["Development and application of sub\u20102\u2010\u03bcm particle CO\n                    <sub>2<\/sub>\n                    \u2010based chromatography coupled to mass spectrometry for comprehensive analysis of lipids in cottonseed extracts"],"prefix":"10.1002","volume":"31","author":[{"given":"Carolina","family":"Salazar","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Jones","sequence":"additional","affiliation":[{"name":"Analytical and Environmental Sciences, School of Biomedical Sciences King's College  London SE1 9NH UK"},{"name":"Waters Corporation  34 Maple Street Milford MA 01757 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drew","family":"Sturtevant","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Horn","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janna","family":"Crossley","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadiza","family":"Zaman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kent D.","family":"Chapman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Wrona","sequence":"additional","affiliation":[{"name":"Waters Corporation  34 Maple Street Milford MA 01757 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgis","family":"Isaac","sequence":"additional","affiliation":[{"name":"Waters Corporation  34 Maple Street Milford MA 01757 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman W.","family":"Smith","sequence":"additional","affiliation":[{"name":"Analytical and Environmental Sciences, School of Biomedical Sciences King's College  London SE1 9NH UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7306-6340","authenticated-orcid":false,"given":"Vladimir","family":"Shulaev","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, College of Arts and Sciences The University of North Texas  Denton TX 76203 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,2,23]]},"reference":[{"key":"e_1_2_6_2_1","volume-title":"Bailey's Industrial Oil and Fat Products","author":"O'Brien R. 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