{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:22:17Z","timestamp":1777605737769,"version":"3.51.4"},"reference-count":54,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,5,15]],"date-time":"2017-05-15T00:00:00Z","timestamp":1494806400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001736","name":"German-Israeli Foundation for Scientific Research and Development","doi-asserted-by":"publisher","award":["1139\/2011"],"award-info":[{"award-number":["1139\/2011"]}],"id":[{"id":"10.13039\/501100001736","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["45\/14"],"award-info":[{"award-number":["45\/14"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2017,6,15]]},"abstract":"\n Rationale<\/jats:title>\n Stable isotope tracers are commonly used to track the transformations of organic carbon (C) and nitrogen (N) in soils but they have not been used to follow coupled cycles of phosphorus (P) and C because P has only one stable isotope.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n A novel dually labeled substrate, composed of a\n 13<\/jats:sup>\n C\u2010labeled glucose backbone attached to a phosphate group with known \u03b4\n 18<\/jats:sup>\n O\n P<\/jats:sub>\n value, was used to follow the fate of C and P derived from the same compound. The substrate was amended to soils from two natural oak forests, differing in their P levels, and the \u03b4\n 13<\/jats:sup>\n C values of respired CO\n 2<\/jats:sub>\n and the \u03b4\n 18<\/jats:sup>\n O\n P<\/jats:sub>\n values of soil bioavailable P were measured.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The \u03b4\n 18<\/jats:sup>\n O\n P<\/jats:sub>\n values of the phosphate accumulated in the soil deviated from the expected value of P that is released from phosphomonoesters by enzymatic reactions (3.7 \u2030) and were driven towards isotopic equilibrium with soil water (21.1 \u2030), a process which is produced as part of microbial metabolism. This suggests that the substrate's P passed through the microbial biomass before accumulating in the soil, reflecting the rapid microbial turnover rate of the added P. A higher fraction of the substrate\u2010C was respired in the higher P soil by the end of the incubation (20.3 % in comparison with 9.1 % in the lower P soil), indicating stronger energy limitation in the higher P soils. The higher\n 13<\/jats:sup>\n C glucose respiration in the higher P soil also triggered a stronger priming effect than in the lower P soil.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our approach demonstrates that\n 13<\/jats:sup>\n C\u2010 and P\u2010\n 18<\/jats:sup>\n O\u2010dually labeled tracers provide invaluable data on the fate of organic P and C in soils. We encourage biogeochemists and soil scientists to develop similar tracers of this kind. Copyright \u00a9 2017 John Wiley & Sons, Ltd.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1002\/rcm.7863","type":"journal-article","created":{"date-parts":[[2017,3,31]],"date-time":"2017-03-31T02:25:31Z","timestamp":1490927131000},"page":"969-977","source":"Crossref","is-referenced-by-count":10,"title":["Use of\n 13<\/sup>\n C\u2010 and phosphate\n 18<\/sup>\n O\u2010labeled substrate for studying phosphorus and carbon cycling in soils: a proof of concept"],"prefix":"10.1002","volume":"31","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8425-8508","authenticated-orcid":false,"given":"Avner","family":"Gross","sequence":"first","affiliation":[{"name":"The Institute of Earth Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alon","family":"Angert","sequence":"additional","affiliation":[{"name":"The Institute of Earth Sciences The Hebrew University of Jerusalem Jerusalem Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,5,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00103"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00022"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006247623877"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1890\/03-8002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10533-010-9466-x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1890\/06-1847.1"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/ele.12269"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2486.2010.02261.x"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2009.02.031"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj2013.05.0186dgs"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soilbio.2015.06.015"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gca.2015.03.023"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.2136\/sssaj2015.06.0219"},{"key":"e_1_2_7_15_1","doi-asserted-by":"crossref","first-page":"3155","DOI":"10.1016\/S0021-9258(19)44021-0","article-title":"Occurrence and characteristics of 18O exchange reactions ratalyzed by sodium\u2010 and potassium\u2010dependent adenosine triphosphatases","volume":"248","author":"Dahms A. 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