{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T03:11:09Z","timestamp":1777518669763,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2017,6,5]],"date-time":"2017-06-05T00:00:00Z","timestamp":1496620800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2017,7,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>In the determination of immunosuppressive drugs cyclosporine A (CSA), tacrolimus (TARO), sirolimus (SIRO), and everolimus (EVE) in whole blood there is an open debate about which is the best assay between immunochemistry and liquid chromatography\/tandem mass spectrometry (LC\/MS\/MS). This work is aimed to explore this topic, focusing on the use of updated assays and the analysis of a large number of samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A certified\n                      <jats:italic>in vitro<\/jats:italic>\n                      diagnostic kit coupled with a medical device LC\/MS\/MS was validated and applied to the analysis of 1192 blood samples of patients treated with immunosuppressive drugs. The results were compared with those obtained by immunoassays.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LC\/MS\/MS approach was found to provide linear, stable, precise, and accurate results, with lower limits of quantification of 12.5, 1.1, 1.2, and 1.2\u00a0\u03bcg\/L for CSA, TACRO, SIRO, and EVE, respectively. With this method 80 samples were analysed and reported within a single work shift. A correlation was observed between the LC\/MS\/MS and immunoassay data, with Spearman correlation coefficients of 0.980 (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0260) for CSA, 0.836 for TACRO (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0562), 0.898 for SIRO (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0113), and 0.904 for EVE (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a0257). Passing\u2010Bablock regression showed the presence of constant and proportional biases for most of the drugs. A Blond\u2010Altman graph showed differences between the assays, with immunoassays generally overestimating the drugs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The LC\/MS\/MS certified kit was validated for the detection of immunosuppressant drugs in whole blood and it provided a high\u2010throughput method that is consistent with the requirements of clinical laboratories. The comparison of patient data between LC\/MS\/MS and up\u2010dated immunoassays shows that a significant discrepancy still exists, especially for CSA and SIRO, confirming the greater specificity associated with use of the LC\/MS\/MS assay Copyright \u00a9 2017 John Wiley &amp; Sons, Ltd.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.7887","type":"journal-article","created":{"date-parts":[[2017,4,24]],"date-time":"2017-04-24T21:02:54Z","timestamp":1493067774000},"page":"1111-1120","source":"Crossref","is-referenced-by-count":23,"title":["Immunosuppressive drugs in whole blood: validation of a commercially available liquid chromatography\/tandem mass spectrometry kit and comparison with immunochemical assays"],"prefix":"10.1002","volume":"31","author":[{"given":"Elisa","family":"Polledri","sequence":"first","affiliation":[{"name":"EPIGET \u2013 Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health Universit\u00e0 degli Studi di Milano and Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"Mercadante","sequence":"additional","affiliation":[{"name":"EPIGET \u2013 Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health Universit\u00e0 degli Studi di Milano and Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chiara","family":"Ferraris Fusarini","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Microbiology Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"Maiavacca","sequence":"additional","affiliation":[{"name":"Department of Clinical Chemistry and Microbiology Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0287-7338","authenticated-orcid":false,"given":"Silvia","family":"Fustinoni","sequence":"additional","affiliation":[{"name":"EPIGET \u2013 Epidemiology, Epigenetics, and Toxicology Lab, Department of Clinical Sciences and Community Health Universit\u00e0 degli Studi di Milano and Fondazione IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico  Milan Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,6,5]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0161-5890(03)00075-0"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199103073241005"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-200008270-00027"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0149-2918(02)85038-X"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2015.12.027"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1592\/phco.30.9.954"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/FTD.0000000000000205"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2016.01.031"},{"key":"e_1_2_7_10_1","volume-title":"ACMIA Cyclosporine 2013\u201004\u201005 C PN 717089.002 [package insert]","year":"2013"},{"key":"e_1_2_7_11_1","volume-title":"ACMIA CSA extended range 2014\u201004\u201014 B PN 781108.001 [package insert]","year":"2014"},{"key":"e_1_2_7_12_1","volume-title":"ACMIA Tacrolimus 2014\u201009\u201003 C PN 717207.001 [package insert]","year":"2014"},{"key":"e_1_2_7_13_1","volume-title":"ACMIA Sirolimus REF 1L76\/B1L761 G4\u20105922\/R08 [package insert]","year":"2013"},{"key":"e_1_2_7_14_1","volume-title":"QMS(R) Everolimus 0160060\u2010F [package insert]","year":"2015"},{"key":"e_1_2_7_15_1","volume-title":"MassTox Immunosupressants in Whole Blood \u2013 OneMinute Test [package insert]","year":"2014"},{"key":"e_1_2_7_16_1","unstructured":"Food and Drug Administration (FDA).Guidance for Industry\u2010Bioanalytical Method 2001. 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