{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T08:12:49Z","timestamp":1785831169414,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01GM106016\u201003"],"award-info":[{"award-number":["1R01GM106016\u201003"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2018,1,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Desorption electrospray ionization mass spectrometry (DESI\u2010MS) has demonstrated utility in differentiating tumor from adjacent normal tissue in both urologic and neurosurgical specimens. We sought to evaluate if this technique had similar accuracy in differentiating oral tongue squamous cell carcinoma (SCC) from adjacent normal epithelium due to current issues with late diagnosis of SCC in advanced stages.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fresh frozen samples of SCC and adjacent normal tissue were obtained by surgical resection. Resections were analyzed using DESI\u2010MS sometimes by a blinded technologist. Normative spectra were obtained for separate regions containing SCC or adjacent normal epithelium. Principal Component Analysis and Linear Discriminant Analysis (PCA\u2010LDA) of spectra were used to predict SCC versus normal tongue epithelium. Predictions were compared with pathology to assess accuracy in differentiating oral SCC from adjacent normal tissue.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Initial PCA score and loading plots showed clear separation of SCC and normal epithelial tissue using DESI\u2010MS. PCA\u2010LDA resulted in accuracy rates of 95% for SCC versus normal and 93% for SCC, adjacent normal and normal. Additional samples were blindly analyzed with PCA\u2010LDA pixel\u2010by\u2010pixel predicted classifications as SCC or normal tongue epithelial tissue and compared against histopathology. The\n                      <jats:italic>m\/z<\/jats:italic>\n                      700\u2013900 prediction model showed a 91% accuracy rate.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>DESI\u2010MS accurately differentiated oral SCC from adjacent normal epithelium. Classification of all typical tissue types and pixel predictions with additional classifications should increase confidence in the validation model.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8019","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T17:06:36Z","timestamp":1509123996000},"page":"133-141","source":"Crossref","is-referenced-by-count":22,"title":["Feasibility of desorption electrospray ionization mass spectrometry for diagnosis of oral tongue squamous cell carcinoma"],"prefix":"10.1002","volume":"32","author":[{"given":"Cedric","family":"D'Hue","sequence":"first","affiliation":[{"name":"Department of Chemistry Purdue University  560 Oval Drive West Lafayette IN 47907\u20102084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Moore","sequence":"additional","affiliation":[{"name":"Comprehensive Cancer Center, Department of Otolaryngology\u2010Head and Neck Surgery University of California at Davis  2521 Stockton Blvd., Suite 7200 Sacramento CA 95817 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don\u2010John","family":"Summerlin","sequence":"additional","affiliation":[{"name":"Indiana University Health Pathology Laboratory  350 West 11th Street, Room 4022 Indianapolis IN 46202\u20104108 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Jarmusch","sequence":"additional","affiliation":[{"name":"Department of Chemistry Purdue University  560 Oval Drive West Lafayette IN 47907\u20102084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clint","family":"Alfaro","sequence":"additional","affiliation":[{"name":"Department of Chemistry Purdue University  560 Oval Drive West Lafayette IN 47907\u20102084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avinash","family":"Mantravadi","sequence":"additional","affiliation":[{"name":"Indiana University Department of Otolaryngology\u2010Head and Neck Surgery  550 N. 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Graham","family":"Cooks","sequence":"additional","affiliation":[{"name":"Department of Chemistry Purdue University  560 Oval Drive West Lafayette IN 47907\u20102084 USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/0284185115587734"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3758-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12291-011-0149-8"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2007.06.011"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0360-3016(89)90126-0"},{"issue":"5","key":"e_1_2_7_7_1","article-title":"Diagnostic aids in the screening of oral cancer","volume":"1","author":"Fedele S","year":"2009","journal-title":"Head Neck Oncol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(03)00041-1"},{"key":"e_1_2_7_9_1","first-page":"51","article-title":"The use of toluidine blue in the diagnosis of neoplastic lesions of the oral cavity","volume":"94","author":"Barrellier P","year":"1993","journal-title":"Rev Stomatol Chir Maxillofac"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1117\/1.2193157"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN02039A"},{"key":"e_1_2_7_12_1","first-page":"339","article-title":"Histologic subtypes of oral squamous cell carcinoma: Prognostic relevance","volume":"73","author":"Pereira MC","year":"2007","journal-title":"J Can Dent Assoc"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bfgp\/elp004"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.11.017"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2011.05.006"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215687110"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1706459114"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201001692"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2006.08.003"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.11"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2014.08.001"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2010.03.028"},{"key":"e_1_2_7_23_1","doi-asserted-by":"crossref","first-page":"5953","DOI":"10.1002\/anie.201001452","article-title":"Discrimination of human astrocytoma subtypes by lipid analysis using desorption electrospray ionization imaging mass spectrometry","volume":"49","author":"Eberlin LS","journal-title":"Angew Chem Int Ed 2010"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2006.04.001"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2007.01395.x"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12276731"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-016-1506-x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.4103\/0970-9371.86342"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN00548A"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN01334E"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.8019","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T14:43:42Z","timestamp":1761489822000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.8019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,12,18]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,1,30]]}},"alternative-id":["10.1002\/rcm.8019"],"URL":"https:\/\/doi.org\/10.1002\/rcm.8019","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,12,18]]}}}