{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T06:11:44Z","timestamp":1771481504885,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T00:00:00Z","timestamp":1546473600000},"content-version":"am","delay-in-days":329,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2018,2,8]],"date-time":"2018-02-08T00:00:00Z","timestamp":1518048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000015","name":"U.S. Department of Energy","doi-asserted-by":"publisher","award":["DE\u2010AC05\u201000OR22725"],"award-info":[{"award-number":["DE\u2010AC05\u201000OR22725"]}],"id":[{"id":"10.13039\/100000015","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2018,3,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Laser microdissection\u2010liquid vortex capture\/electrospray ionization mass spectrometry (LMD\u2010LVC\/ESI\u2010MS) has potential for on\u2010line classification of tissue but an investigation into what analytical conditions provide best spectral differentiation has not been conducted. The effects of solvent, ionization polarity, and spectral acquisition parameters on differentiation of mouse brain tissue regions are described.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Individual 40 \u00d7 40\u00a0\u03bcm microdissections from cortex, white, grey, granular, and nucleus regions of mouse brain tissue were analyzed using different capture\/ESI solvents, in positive and negative ion mode ESI, using time\u2010of\u2010flight (TOF)\u2010MS and sequential window acquisitions of all theoretical spectra (SWATH)\u2010MS (a permutation of tandem\u2010MS), and combinations thereof. Principal component analysis\u2010linear discriminant analysis (PCA\u2010LDA), applied to each mass spectral dataset, was used to determine the accuracy of differentiation of mouse brain tissue regions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Mass spectral differences associated with capture\/ESI solvent composition manifested as altered relative distributions of ions rather than the presence or absence of unique ions. In negative ion mode ESI, 80\/20 (v\/v) methanol\/water yielded spectra with low signal\/noise ratios relative to other solvents. PCA\u2010LDA models acquired using 90\/10 (v\/v) methanol\/chloroform differentiated tissue regions with 100% accuracy while data collected using methanol misclassified some samples. The combination of SWATH\u2010MS and TOF\u2010MS data improved differentiation accuracy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Combined TOF\u2010MS and SWATH\u2010MS data differentiated white, grey, granular, and nucleus mouse tissue regions with greater accuracy than when solely using TOF\u2010MS data. Using 90\/10 (v\/v) methanol\/chloroform, tissue regions were perfectly differentiated. These results will guide future studies looking to utilize the potential of LMD\u2010LVC\/ESI\u2010MS for tissue and disease differentiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8053","type":"journal-article","created":{"date-parts":[[2018,1,3]],"date-time":"2018-01-03T06:13:27Z","timestamp":1514960007000},"page":"414-422","source":"Crossref","is-referenced-by-count":11,"title":["Solvent effects on differentiation of mouse brain tissue using laser microdissection \u2018cut and drop\u2019 sampling with direct mass spectral analysis"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9866-4010","authenticated-orcid":false,"given":"John F.","family":"Cahill","sequence":"first","affiliation":[{"name":"Mass Spectrometry and Laser Spectroscopy Group, Chemical Sciences Division Oak Ridge National Laboratory  Oak Ridge TN 37831\u20106131 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0186-5797","authenticated-orcid":false,"given":"Vilmos","family":"Kertesz","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Laser Spectroscopy Group, Chemical Sciences Division Oak Ridge National Laboratory  Oak Ridge TN 37831\u20106131 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5454-1863","authenticated-orcid":false,"given":"Tiffany","family":"Porta","sequence":"additional","affiliation":[{"name":"Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry Maastricht University  Universiteitssingel 50 6229 ER Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.C. Yves","family":"LeBlanc","sequence":"additional","affiliation":[{"name":"Sciex  Concord Ontario Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-7179","authenticated-orcid":false,"given":"Ron M.A.","family":"Heeren","sequence":"additional","affiliation":[{"name":"Maastricht MultiModal Molecular Imaging (M4I) Institute, Division of Imaging Mass Spectrometry Maastricht University  Universiteitssingel 50 6229 ER Maastricht The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5224-3969","authenticated-orcid":false,"given":"Gary J.","family":"Van Berkel","sequence":"additional","affiliation":[{"name":"Mass Spectrometry and Laser Spectroscopy Group, Chemical Sciences Division Oak Ridge National Laboratory  Oak Ridge TN 37831\u20106131 USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,2,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3295"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.237172"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/bs.acr.2016.11.011"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijms.2014.06.024"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1215687110"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002108"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1400274111"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1227\/NEU.0b013e3181ff9cbb"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-2465"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1404724111"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1523306113"},{"issue":"26","key":"e_1_2_6_13_1","doi-asserted-by":"crossref","first-page":"6700","DOI":"10.1073\/pnas.1706459114","article-title":"Intraoperative assessment of tumor margins during glioma resection by desorption electrospray ionization\u2010mass spectrometry","volume":"114","author":"Pirro V","year":"2017","journal-title":"Proc Natl Acad Sci"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN00548A"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1039\/C4AN02039A"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac9029482"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200502362"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2008.12.058"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1408129111"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-14-2258"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201001452"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-011-0134-8"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-010-4259-6"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201001692"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409778111"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-012-5841-x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3CC48927B"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1039\/C7AN00830A"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.8019"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1039\/C3AN02340K"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361-012-0462-3"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3an36554a"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005623"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac5046752"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep25919"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac102613m"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aan3968"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac201251s"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1039\/c2an36006c"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b03293"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7477"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.6b01155"},{"issue":"6","key":"e_1_2_6_43_1","first-page":"559","article-title":"Interinstitutional comparison of frozen section turnaround time. 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