{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:21:04Z","timestamp":1790162464726,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T00:00:00Z","timestamp":1521936000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2018,4,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Drug discovery studies invariably require qualitative and quantitative analyses of target compounds at every stage of drug discovery. We have developed a system combining molecular interaction analysis and mass spectrometry (LC\u2010MS) using the principle of nanopore optical interferometry (nPOI) called molecular interaction kinetics\u2013mass spectrometry (MIK\u2010MS). Since nPOI has high binding capacity, the bond\u2010dissociated compound can be directly detected using LC\u2010MS. In this study, we use carbonic anhydrase II (CAII) as a ligand and apply six small compounds as analytes and report the affinity analysis using MIK\u2010MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>CAII was immobilized onto a COOH sensor chip using standard amine coupling. A reference surface was prepared by activating and subsequently blocking the surface under identical conditions. An amount of 50 \u03bcL of mix solution was injected over the reference channel and sample channel for CAII immobilization. The solutions eluting from the sensor chip were collected from the waste\u2010line of the SKi Pro system every 30\u00a0s. Reconstructed elution samples were then injected into the LC\u2010MS\/MS system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A mixture containing furosemide, acetazolamide, 4\u2010sulfamoylbenzoic acid, 5\u2010(dimethylamino)\u20101\u2010naphthalene sulfonamide (DNSA), sulfanilamide and sulpiride (15 \u03bcM each) was injected into the CAII\u2010immobilized sensor chip, and the fractions eluted from the SKi Pro system were collected and subjected to selected reaction monitoring LC\u2010MS characterization. Specific results were obtained for acetazolamide, DNSA, furosemide and sulpiride. The results suggest that the association\u2013dissociation curve of a mixed sample can be obtained by one\u2010time MIK\u2010MS analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Six small\u2010molecule binders of CAII were analyzed quantitatively using nPOI and MIK\u2010MS, and the results were compared to published surface plasmon resonance (SPR) results. The nPOI and SPR results show good agreement, confirming the reliability of the analysis. Time\u2010dependent binding results may be obtained by our MS sensorgram approach. Drugs that meet medical needs in a short period are required; this nPOI\u2010LC\u2010MS system is considered an important tool for rapid drug discovery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8083","type":"journal-article","created":{"date-parts":[[2018,2,14]],"date-time":"2018-02-14T01:36:43Z","timestamp":1518572203000},"page":"665-671","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Development of drug discovery screening system by molecular interaction kinetics\u2013mass spectrometry"],"prefix":"10.1002","volume":"32","author":[{"given":"Naoko","family":"Obi","sequence":"first","affiliation":[{"name":"Nissha Co. Ltd  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Biosys Technologies Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noboru","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Biosys Technologies Inc.  Tokyo Japan"},{"name":"Translational Medicine Informatics St Marianna University School of Medicine, Research &amp; Development, Biosys Technologies Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ervin","sequence":"additional","affiliation":[{"name":"Silicon Kinetics Inc.  San Diego CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhiko","family":"Bando","sequence":"additional","affiliation":[{"name":"Biosys Technologies Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihide","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Biosys Technologies Inc.  Tokyo Japan"},{"name":"Translational Medicine Informatics St Marianna University School of Medicine, Research &amp; Development, Biosys Technologies Inc.  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Nagatoishi","sequence":"additional","affiliation":[{"name":"Institute of Medical Science The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kouhei","family":"Tsumoto","sequence":"additional","affiliation":[{"name":"School of Engineering The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1613-6968","authenticated-orcid":false,"given":"Takeshi","family":"Kawamura","sequence":"additional","affiliation":[{"name":"Proteomics Laboratory, Isotope Science Center The University of Tokyo  Tokyo Japan"},{"name":"Laboratories for Systems Biology and Medicine (LSBM), Research Center for Advanced Science and Technology (RCAST) The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2011.34.7146"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-014-0070-8"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1382"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-0776"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2147\/DDDT.S19045"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dmpk.2015.11.008"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.02.027"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2006.08.021"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.84"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200401353"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2008.06.030"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(01)01797-8"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr050132x"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5207-9"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac015642z"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-014-8373-8"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1255\/ejms.448"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200600065"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-5956-6-31"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5339.840"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2015.06.002"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.11.011"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2009.11.010"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176219"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2014.12.029"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2004.03.028"},{"key":"e_1_2_6_28_1","unstructured":"Silicon Kinetics Application Note 11 Characterization of small molecule to proteinhttp:\/\/www.siliconkinetics.com. Accessed January 31 2018."}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.8083","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8083","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:12:06Z","timestamp":1761484326000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.8083"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,25]]},"references-count":27,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,4,30]]}},"alternative-id":["10.1002\/rcm.8083"],"URL":"https:\/\/doi.org\/10.1002\/rcm.8083","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,25]]},"assertion":[{"value":"2017-08-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-02-02","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}