{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:19:20Z","timestamp":1790194760401,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2018,10,4]],"date-time":"2018-10-04T00:00:00Z","timestamp":1538611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["IZS ME 05\/14 RC"],"award-info":[{"award-number":["IZS ME 05\/14 RC"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2018,11,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Carry\u2010over is an undesirable contamination from medicated to non\u2010medicated during the production of feedingstuffs. In 2014 the European Parliament and the Council started working to produce a new regulatory act that will fix tolerable levels of drugs by carry\u2010over in non\u2010target feed to have a harmonized practice to evaluate this contamination by veterinary drugs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We developed a rapid and effective multi\u2010analyte method coupling ultraperformance liquid chromatography to tandem mass spectrometry (UPLC\/MS\/MS) for the detection of 37 drugs belonging to different classes of antimicrobials (sulfonamides, tetracyclines, macrolides, quinolones, pleuromutilins and streptogramins) in feeds at carry\u2010over levels. The method was in\u2010house validated in the concentration range 0.25\u20132.0\u00a0mg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      , according to the Regulation (UE) 2017\/625 requirements and the guideline included in the Commission Decision 2002\/657\/EC for official methods.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The UPLC\/MS\/MS method allows the determination of the antimicrobials in 15\u00a0min, by providing results compliant to the criteria established by the European Commission legislation. All the analytes showed a limit of detection (LOD) in the range 2.0\u20135.0\u00a0\u03bcg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      and a limit of quantification (LOQ) at 10.0\u00a0\u03bcg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      ; oxytetracycline, doxycycline, spiramycin and virginiamycin have a higher LOD and LOQ (15.0\u00a0\u03bcg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      ; 30.0\u00a0\u03bcg\u00a0kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      , respectively). Recoveries were satisfactory ranging from 90.4% to 103.1%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The method is characterized by an effective clean\u2010up of all drugs without the use of large sample size and organic solvent extraction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8216","type":"journal-article","created":{"date-parts":[[2018,6,26]],"date-time":"2018-06-26T00:20:28Z","timestamp":1529972428000},"page":"1831-1842","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Multi\u2010drug ultraperformance liquid chromatography\/tandem mass spectrometry method to quantify antimicrobials in feeding stuffs at carry\u2010over level"],"prefix":"10.1002","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0251-4331","authenticated-orcid":false,"given":"Cinzia","family":"Civitareale","sequence":"first","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health Istituto Superiore di Sanit\u00e0  Viale Regina Elena 299 00161 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serena","family":"Mestria","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health Istituto Superiore di Sanit\u00e0  Viale Regina Elena 299 00161 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9676-2290","authenticated-orcid":false,"given":"Pasquale","family":"Gallo","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale del Mezzogiorno  Via Salute 2 80055 Portici (Na) Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luigi","family":"Giannetti","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Regioni Lazio e Toscana  Via Appia Nuova 1411 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Neri","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale Regioni Lazio e Toscana  Via Appia Nuova 1411 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Stacchini","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health Istituto Superiore di Sanit\u00e0  Viale Regina Elena 299 00161 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maurizio","family":"Fiori","sequence":"additional","affiliation":[{"name":"Department of Food Safety, Nutrition and Veterinary Public Health Istituto Superiore di Sanit\u00e0  Viale Regina Elena 299 00161 Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,10,4]]},"reference":[{"key":"e_1_2_6_2_1","unstructured":"Council Directive 90\/167\/EEC of 26 March 1990 laying down the conditions governing the preparation placing on the market and use of medicated feedingstuffs in the Community."},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(02)00751-1"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00320-4"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2011.02.012"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anifeedsci.2006.08.005"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2011.569574"},{"key":"e_1_2_6_8_1","first-page":"1","article-title":"Cross\u2010contamination of non\u2010target feedingstuffs by decoquinate authorised for use as a feed 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