{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T16:08:47Z","timestamp":1784736527671,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2019,2,15]],"date-time":"2019-02-15T00:00:00Z","timestamp":1550188800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG\u2010FOR 2337"],"award-info":[{"award-number":["DFG\u2010FOR 2337"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Field measurement of denitrification in agricultural ecosystems using the\n                      <jats:sup>15<\/jats:sup>\n                      N gas flux method has been limited by poor sensitivity because current isotope ratio mass spectrometry is not precise enough to detect low\n                      <jats:sup>15<\/jats:sup>\n                      N\n                      <jats:sub>2<\/jats:sub>\n                      fluxes in the presence of a high atmospheric N\n                      <jats:sub>2<\/jats:sub>\n                      background. For laboratory studies, detection limits are improved by incubating soils in closed systems and under N\n                      <jats:sub>2<\/jats:sub>\n                      \u2010depleted atmospheres.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We developed a new procedure to conduct the\n                      <jats:sup>15<\/jats:sup>\n                      N gas flux method suitable for field application using an artificially N\n                      <jats:sub>2<\/jats:sub>\n                      \u2010depleted atmosphere to improve the detection limit at the given precision of mass spectrometry. Laboratory experiments with and without\n                      <jats:sup>15<\/jats:sup>\n                      N\u2010labelling and using different flushing strategies were conducted to develop a suitable field method. Subsequently, this method was tested in the field and results were compared with those obtained from the conventional\n                      <jats:sup>15<\/jats:sup>\n                      N gas flux method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Results of the two methods were in close agreement showing that the denitrification rates determined were not biased by the flushing procedure. Best sensitivity for N\n                      <jats:sub>2<\/jats:sub>\n                      \u2009+\u2009N\n                      <jats:sub>2<\/jats:sub>\n                      O fluxes was 10\u2009ppb, which was 80\u2010fold better than that of the reference method. Further improvement can be achieved by lowering the N\n                      <jats:sub>2<\/jats:sub>\n                      background concentration below the values established in the present study.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In view of this progress in sensitivity, the new method will be suitable to measure denitrification dynamics in the field beyond peak events.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8363","type":"journal-article","created":{"date-parts":[[2018,11,25]],"date-time":"2018-11-25T20:09:20Z","timestamp":1543176560000},"page":"437-448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Improvement of the\n                    <sup>15<\/sup>\n                    N gas flux method for\n                    <i>in situ<\/i>\n        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