{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T18:29:04Z","timestamp":1793125744177,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T00:00:00Z","timestamp":1552348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Xinglin Youth Scholar Funded Scheme","award":["A1-2606-18-414-010"],"award-info":[{"award-number":["A1-2606-18-414-010"]}]},{"name":"Xinglin Youth Scholar Funded Scheme","award":["AFD018181Z3938"],"award-info":[{"award-number":["AFD018181Z3938"]}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2019,4,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Hexafluoroisopropanol (HFIP) has been widely used as a counter anion in the mobile phase for ion\u2010pairing reversed\u2010phase liquid chromatography\/mass spectrometry (IP\u2010RP\u2010LC\/MS) analysis of oligonucleotides. However, researchers are still searching for improvements to counter anions for LC\/MS analysis of oligonucleotides. This study aimed to find alternatives to HFIP for analyzing oligonucleotides.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The study was performed using an Agilent 1290 ultra\u2010high\u2010performance liquid chromatography (UHPLC) system coupled to an Agilent 6540 mass spectrometer by using an oligonucleotide BEH C18 column (100\u2009\u00d7\u20092.1\u2009mm, 1.7\u2009\u03bcm). Buffer systems containing ion\u2010pairing reagents (triethylamine, tripropylamine, hexylamine, dimethylbutylamine, diisopropylethylamine,\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010dimethylcyclohexylamine, and octylamine) and fluoroalcohols (HFIP and hexafluoro\u20102\u2010methyl\u20102\u2010propanol (HFTP)) were compared chromatographically and mass spectrometrically.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Results showed that HFTP has better desalting ability than HFIP, but both HFIP and HFTP have comparable effects on the separation of oligonucleotides sized from 10mer to 40mer for most of ion\u2010pairing reagents, with the exception of triethylamine and\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010dimethylcyclohexylamine, where HFIP performed better than HFTP.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The choice of fluoroalcohols in IP\u2010RP\u2010LC\/MS analysis of oligonucleotides depends on the type of ion\u2010pairing reagents used in the mobile phase. As a guideline, we would recommend to use either HA\u2010HFIP or HA\u2010HFTP for small oligonucleotides, but TPA\u2010HFTP for large oligonucleotides for IP\u2010RP\u2010LC\/MS analysis of synthetic oligonucleotides.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8386","type":"journal-article","created":{"date-parts":[[2019,1,23]],"date-time":"2019-01-23T22:57:12Z","timestamp":1548284232000},"page":"697-709","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["The role of fluoroalcohols as counter anions for ion\u2010pairing reversed\u2010phase liquid chromatography\/high\u2010resolution electrospray ionization mass spectrometry analysis of oligonucleotides"],"prefix":"10.1002","volume":"33","author":[{"given":"Rong","family":"Liu","sequence":"first","affiliation":[{"name":"Joint Laboratory for Translational Cancer Research of Chinese Medicine of the Ministry of Education of the People's Republic of 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