{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,7]],"date-time":"2026-04-07T06:14:56Z","timestamp":1775542496176,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T00:00:00Z","timestamp":1574294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2019,12,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>We developed a new high\u2010throughput method to analyze tegafur (FT) and 5\u2010fluorouracil (5\u2010FU) in tear and plasma samples using hydrophilic interaction liquid chromatography (HILIC)\/tandem mass spectrometry (MS\/MS).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The tear samples (10\u2009\u03bcL) spiked with FT, 5\u2010FU, and 5\u2010chlorouracil (internal standard) were diluted using 40\u2009\u03bcL of 2\u2009M ammonium acetate and 250\u2009\u03bcL of acetonitrile with 2% formic acid; 20\u2009\u03bcL of plasma spiked with the two drugs and internal standard was diluted with 80\u2009\u03bcL of 2\u2009M ammonium acetate and 500\u2009\u03bcL of acetonitrile with 2% formic acid. After centrifugation, the clear supernatant extract (15\u2009\u03bcL) was directly injected into the HILIC\/MS\/MS instrument, and each drug was separated on a Unison UK\u2010Amino column (50\u2009mm\u2009\u00d7\u20093\u2009mm i.d., 3\u2009\u03bcm particle size) with a linear gradient elution system composed of 10\u2009mM ammonium acetate (pH\u20096.8) and acetonitrile at a flow rate of 0.7\u2009mL\/min. We performed quantification by multiple reaction monitoring (MRM) with negative\u2010ion atmospheric\u2010pressure chemical ionization.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Distinct peaks were observed for the drugs on each MRM channel within 2\u2009min. The regression equations showed good linearity within the range 0.04\u20134.0\u2009\u03bcg\/mL for the tear and plasma samples with detection limits at 0.02\u20130.04\u2009\u03bcg\/mL. Recoveries for target analytes (FT and 5\u2010FU) for the tear and plasma samples were in the 94\u2013128% and 94\u2013104% ranges, respectively. The intra\u2010 and inter\u2010day coefficients of variation for the two drugs were lower than 10.8%. The accuracies of quantitation were 97\u2013115% for both samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We established a high\u2010throughput, reproducible, and practical procedure for analyzing FT and 5\u2010FU in human tear and plasma samples using HILIC\/MS\/MS analysis with an aminopropyl\u2010bonded mixed\u2010mode separation column. This method can be applied to the high\u2010throughput routines used in clinical analyses.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8531","type":"journal-article","created":{"date-parts":[[2019,7,19]],"date-time":"2019-07-19T18:48:36Z","timestamp":1563562116000},"page":"1906-1914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010throughput method to analyze tegafur and 5\u2010fluorouracil in human tears and plasma using hydrophilic interaction liquid chromatography\/tandem mass spectrometry"],"prefix":"10.1002","volume":"33","author":[{"given":"Ritsuko","family":"Shiokawa","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Showa University School of Medicine  1\u20105\u20108 Hatanodai, Shinagawa\u2010ku Tokyo 142\u20108555 Japan"},{"name":"Department of Legal Medicine Showa University School of Medicine  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