{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T16:43:01Z","timestamp":1761496981455,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,8]],"date-time":"2020-01-08T00:00:00Z","timestamp":1578441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013076","name":"National Major Science and Technology Projects of China","doi-asserted-by":"publisher","award":["2017ZX09101001\u2010006\u2010014"],"award-info":[{"award-number":["2017ZX09101001\u2010006\u2010014"]}],"id":[{"id":"10.13039\/501100013076","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2020,1,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Structured triacylglycerols (STAGs) are a complex mixture of triacylglycerols which are esterified by long\u2010chain fatty acids and medium\u2010chain fatty acids with the same glycerol molecular backbone. As a kind of lipid pharmaceutic excipients, STAGs are used in the pharmaceutical industry as major components of structured fat emulsion injections and play an important role in pharmaceutic energy material because they improve nutritional status with faster elimination in a safe way. The composition and proportion of triacylglycerols in STAGs are closely related to the stability of pharmaceutical preparations and curative effects in the clinic. Therefore, it is necessary to characterize pharmaceutic STAGs using a rapid and accurate method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An analytical method for rapid and accurate determination of triacylglycerols in pharmaceutic STAGs was developed using high\u2010performance liquid chromatography\/tandem high\u2010resolution mass spectrometry (HPLC\/HRMS). Triacylglycerol components could be well separated on a Waters Xterra MS C8 (2.1\u2009\u00d7\u2009100\u2009mm, 3.5\u2009\u03bcm) column. Four\u2010dimensional HPLC\/HRMS data (high\u2010resolution\n                      <jats:italic>m\/z<\/jats:italic>\n                      , MS\n                      <jats:sup>2<\/jats:sup>\n                      data, retention time and isotopic intensity distribution) were used to identify triacylglycerols using Lipid Data Analyzer (LDA) software and the LIPID MAPS database. Then, these identified triacylglycerol components were relatively quantified by their corresponding normalized peak areas using representative standard curves of structurally similar standard substances.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Forty\u2010seven kinds of triacylglycerol components in pharmaceutic STAGs and structured fat emulsion injection were identified and relatively quantified by this method. It has been shown that their retention times are in good correlation with the number of carbon atoms and carbon\u2013carbon double bonds. The main components in pharmaceutic STAGs and structured fat emulsion injection were triacylglycerols containing both medium\u2010chain fatty acids and long\u2010chain fatty acids, while the other components, including triacylglycerols containing three medium\u2010chain fatty acids and triacylglycerols containing three long\u2010chain fatty acids, were relatively low.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study has provided a rapid and accurate approach for the identification and quality control of pharmaceutic STAGs and structured fat emulsion injection and this approach can be extended to other lipid pharmaceutic excipients and used as an effective and reasonable control to guarantee the quality of pharmaceutical preparations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8557","type":"journal-article","created":{"date-parts":[[2019,8,19]],"date-time":"2019-08-19T20:59:08Z","timestamp":1566248348000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of pharmaceutic structured triacylglycerols by high\u2010performance liquid chromatography\/tandem high\u2010resolution mass spectrometry and its application to structured fat emulsion injection"],"prefix":"10.1002","volume":"34","author":[{"given":"Hao","family":"Zheng","sequence":"first","affiliation":[{"name":"State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Yang","sequence":"additional","affiliation":[{"name":"National Institute For Food and Drug Control  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhe","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jue","family":"Wang","sequence":"additional","affiliation":[{"name":"National Institute For Food and Drug Control  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6125-7964","authenticated-orcid":false,"given":"Jinlan","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica Chinese Academy of Medical Sciences &amp; Peking Union Medical College  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huimin","family":"Sun","sequence":"additional","affiliation":[{"name":"National Institute For Food and Drug Control  Beijing 100050 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,1,8]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7827(03)00050-X"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.algal.2017.05.025"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607189013006614"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/ajcn\/53.5.1217"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1177\/014860719101500137"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0148607101025005245"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2005.09.002"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1438-9312(200004)102:4<287::AID-EJLT287>3.0.CO;2-Q"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/3527606033.ch8"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.4700"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2011.05.064"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0231(20010215)15:3<187::AID-RCM211>3.0.CO;2-T"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-011-5376-6"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac201195d"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkl838"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm324"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq699"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-013-7479-8"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-0982-x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac060545x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btk039"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep06581"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2015.01.032"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b02023"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b02839"},{"issue":"112","key":"e_1_2_6_27_1","first-page":"1","article-title":"Introduction to fatty acids and lipids","volume":"112","author":"Burdge GC","year":"2015","journal-title":"World Rev Nutr Diet"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.8557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8557","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.8557","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8557","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T16:38:02Z","timestamp":1761496682000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.8557"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,8]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1,15]]}},"alternative-id":["10.1002\/rcm.8557"],"URL":"https:\/\/doi.org\/10.1002\/rcm.8557","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"type":"print","value":"0951-4198"},{"type":"electronic","value":"1097-0231"}],"subject":[],"published":{"date-parts":[[2020,1,8]]},"assertion":[{"value":"2019-06-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e8557"}}