{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T22:22:56Z","timestamp":1774390976053,"version":"3.50.1"},"reference-count":17,"publisher":"Wiley","issue":"S4","license":[{"start":{"date-parts":[[2020,2,11]],"date-time":"2020-02-11T00:00:00Z","timestamp":1581379200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["090937"],"award-info":[{"award-number":["090937"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The activity of the glucocorticoid activating enzyme 11\u03b2\u2010hydroxysteroid dehydrogenase type\u20101 (11\u03b2HSD1) is altered in diseases such as obesity, inflammation and psychiatric disorders. In rodents 11\u03b2HSD1 converts inert 11\u2010dehydrocorticosterone (11\u2010DHC) into the active form, corticosterone (CORT). A sensitive, specific liquid chromatography\/tandem mass spectrometry method was sought to simultaneously quantify total 11\u2010DHC and total and free CORT in murine plasma for simple assessment of 11\u03b2HSD1 activity in murine models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Mass spectrometry parameters were optimised and a method for the chromatographic separation of CORT and 11\u2010DHC was developed. Murine plasma was prepared by 10:1 chloroform liquid\u2013liquid extraction (LLE) for analysis. Limits of quantitation (LOQs), linearity and other method criteria were assessed, according to bioanalytical method validation guidelines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Reliable separation of 11\u2010DHC and CORT was achieved using an ACE Excel 2 C18\u2010AR (2.1\u2009\u00d7\u2009150\u2009mm; 2\u2009\u03bcm) fused core column at 25\u00b0C, with an acidified water\/acetonitrile gradient over 10\u2009min. Analytes were detected by multiple reaction monitoring after positive electrospray ionisation (\n                      <jats:italic>m\/z<\/jats:italic>\n                      345.1.1 \u2794 121.2,\n                      <jats:italic>m\/z<\/jats:italic>\n                      347.1 \u2794 121.1 for 11\u2010DHC and CORT, respectively). The LOQs were 0.25 and 0.20\u2009ng\/mL for 11\u2010DHC and CORT, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This LC\/MS method is suitable for the reliable analysis of 11\u2010DHC and CORT following simple LLE of murine plasma, bringing preclinical analysis in line with recommendations for clinical endocrinology and biochemistry.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8610","type":"journal-article","created":{"date-parts":[[2019,11,2]],"date-time":"2019-11-02T09:54:24Z","timestamp":1572688464000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Quantitative analysis of 11\u2010dehydrocorticosterone and corticosterone for preclinical studies by liquid chromatography\/triple quadrupole mass spectrometry"],"prefix":"10.1002","volume":"34","author":[{"given":"Manu","family":"Verma","sequence":"first","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Sooy","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Core, Edinburgh Clinical Research Facility, Queen's Medical Research Institute University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Just","sequence":"additional","affiliation":[{"name":"Mass Spectrometry Core, Edinburgh Clinical Research Facility, Queen's Medical Research Institute University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark","family":"Nixon","sequence":"additional","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Morgan","sequence":"additional","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruth","family":"Andrew","sequence":"additional","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen E.","family":"Chapman","sequence":"additional","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9262-2098","authenticated-orcid":false,"given":"Natalie Z.M.","family":"Homer","sequence":"additional","affiliation":[{"name":"University\/BHF Centre for Cardiovascular Sciences, Queen's Medical Research Institute, University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ UK"},{"name":"Mass Spectrometry Core, Edinburgh Clinical Research Facility, Queen's Medical Research Institute University of Edinburgh  47 Little France Crescent Edinburgh EH16 4TJ 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