{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T18:10:38Z","timestamp":1773252638102,"version":"3.50.1"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2020,4,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Ferulic acid (FA) is a standard matrix used for analyzing proteins. In this study, the ability of a halogenated FA to serve as an effective MALDI matrix was investigated. Various halogenated FAs were synthesized, and the characteristics and performance of each were compared with those of the standard matrices \u03b1\u2010cyano\u20104\u2010hydroxycinnamic acid (CHCA) and 2,5\u2010dihydrobenzoic acid (DHBA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The abilities of 6\u2010bromoferulic acid (6\u2010BFA), ferulic acid (FA), and eight other halogenated FA derivatives to ionize eight synthetic peptides were examined. Absorption measurements, MM2 structure optimizations, and proton affinity (PA) calculations were also performed for 6\u2010BFA and FA. The suitabilities of these compounds as matrices for matrix\u2010assisted laser desorption\/ionization (MALDI) for lipids, sugar chains, polymers, cyanocobalamin, synthetic peptides, and tryptic peptides originating from two types of serum proteins were also tested.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The 6\u2010position of FA was found to be the best site for introducing a bromine because the generated compound allowed facile detection of cyanocobalamin and several peptides. 6\u2010BFA exhibited good sensitivity for large peptides (3\u20135\u2009kDa) and peptides containing acidic amino acids or proline. 6\u2010BFA was also shown to be a suitable matrix for tandem mass spectrometry (MS\/MS) analysis when using MALDI time\u2010of\u2010flight (TOF) mass spectrometry (MS) with a quadrupole ion trap (QIT) system.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The properties of 6\u2010BFA as a MALDI matrix differed from those of DHBA and CHCA. 6\u2010BFA appears to be a useful matrix for\n                      <jats:italic>de novo<\/jats:italic>\n                      sequencing using MALDI\u2010QIT\u2010TOF\u2010MS.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8636","type":"journal-article","created":{"date-parts":[[2019,10,28]],"date-time":"2019-10-28T23:34:45Z","timestamp":1572305685000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of 6\u2010bromoferulic acid as a novel common\u2010use matrix for matrix\u2010assisted laser desorption\/ionization time\u2010of\u2010flight mass spectrometry"],"prefix":"10.1002","volume":"34","author":[{"given":"Yosuke","family":"Kato","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Narumi","family":"Hirosawa","sequence":"additional","affiliation":[{"name":"Biomedical Research Center Saitama Medical University  38 Morohongo, Moroyama, Iruma\u2010gun Saitama 350\u20100495 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Moriguchi","sequence":"additional","affiliation":[{"name":"Department of Liberal Arts, Faculty of Medicine Saitama Medical University  38 Morohongo, Moroyama, Iruma\u2010gun Saitama 350\u20100495 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Takayama","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meiyan","family":"Xuan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mari","family":"Okazaki","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"Biomedical Research Center Saitama Medical University  38 Morohongo, Moroyama, Iruma\u2010gun Saitama 350\u20100495 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayako","family":"Hoshi","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Sciences Josai University  1\u20101 Keyakidai, Sakado Saitama 350\u20100295 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7289-9156","authenticated-orcid":false,"given":"Yasushi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Biomedical Research Center Saitama Medical University  38 Morohongo, Moroyama, Iruma\u2010gun Saitama 350\u20100495 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