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However, there is little information for tropical forest soils, which presents a challenge for \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      measurements due to low available P concentrations. Here we report the use of a rapid ammonium fluoride extraction method (Bray\u20101) as an alternative to the widely used anion\u2010exchange membrane (AEM) method for quantification of \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      values of available P in tropical forest soils.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We compared P concentrations and \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      values of available and microbial P determined by AEM and Bray\u20101 extraction for a series of tropical forest soils from Panama spanning a steep P gradient. This involved an assessment of the influence of extraction conditions, including temperature, extraction time, fumigation time and solution\u2010to\u2010soil ratio, on P concentrations and isotope ratios.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Depending on the extraction conditions, Bray\u20101 P concentrations ranged from 0.2 to 66.3\u2009mg P kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      across the soils. Extraction time and temperature had only minor effects on Bray\u20101 P, but concentrations increased markedly as the solution\u2010to\u2010soil ratio increased. In contrast, extraction conditions did not affect Bray\u20101 \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      values, indicating that Bray\u20101 provides a robust measure of the isotopic composition of available soil P. For a relatively high P soil, available and fumigation\u2010released (microbial) \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      values determined by Bray\u20101 extraction (20\u2030 and 16\u2030, respectively) were higher than those determined by the AEM method (18\u2030 and 12\u2030, respectively), which we attribute to slightly different P pools extracted by the two methods and\/or differences resulting from the longer extraction time needed for the AEM method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The short extraction time, insensitivity to extraction conditions and smaller mass of soil required to extract sufficient P for isotopic analysis make Bray\u20101extraction a suitable alternative to the AEM method for the determination of \u03b4\n                      <jats:sup>18<\/jats:sup>\n                      O\n                      <jats:sub>P<\/jats:sub>\n                      values of available P in tropical soils.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8647","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T14:04:03Z","timestamp":1572530643000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A rapid ammonium fluoride method to determine the oxygen isotope ratio of available phosphorus in tropical soils"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7610-0484","authenticated-orcid":false,"given":"Verena","family":"Pfahler","sequence":"first","affiliation":[{"name":"Sustainable Agriculture Sciences Rothamsted Research  North Wyke Okehampton EX20 2SB UK"},{"name":"Smithsonian Tropical Research Institute  Apartado 0843\u201003092 Balboa Ancon Republic of Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aleksandra","family":"Bielnicka","sequence":"additional","affiliation":[{"name":"Smithsonian Tropical Research Institute  Apartado 0843\u201003092 Balboa Ancon Republic of Panama"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew C.","family":"Smith","sequence":"additional","affiliation":[{"name":"NERC Isotope Geoscience Laboratory British Geological Survey  Nottingham NG12 5GG UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0183-0244","authenticated-orcid":false,"given":"Steven J.","family":"Granger","sequence":"additional","affiliation":[{"name":"Sustainable Agriculture Sciences Rothamsted Research  North Wyke Okehampton EX20 2SB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin S.A.","family":"Blackwell","sequence":"additional","affiliation":[{"name":"Sustainable Agriculture Sciences Rothamsted Research  North Wyke Okehampton EX20 2SB UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benjamin L.","family":"Turner","sequence":"additional","affiliation":[{"name":"Smithsonian Tropical Research Institute  Apartado 0843\u201003092 Balboa Ancon Republic of 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