{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T01:54:45Z","timestamp":1775008485189,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2020,10,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Formaldehyde (FA) exposure via environmental pollution or through the food chain poses a serious threat to human health, especially in developing countries like India. Although the addition of FA to food is proscribed, it is often illegally added to foods such as milk to increase the shelf\u2010life. There are challenges in differentiating the endogenous FA content in milk from externally added FA.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>We have developed a simple method using ultra\u2010high\u2010performance liquid chromatography\/tandem mass spectrometry in selected reaction monitoring mode (UHPLC\/MS\/SRM) for the absolute quantification of endogenous FA in milk. The steps include fat removal, protein precipitation using acid, and spiking with labelled FA (FA*), followed by simple click chemistry\u2010based derivatization using Girard P reagent (GP) and final analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A standard curve with FA* was constructed and used for the calculation of endogenous FA in milk. The optimal conditions for the derivatization reaction using 500\u2009\u03bcL of milk were: GP, 50\u2009\u03bcg; temperature, 37\u00b0C; time, 60\u2009min; and 0.1% HCl. The validation parameters such as accuracy (95.84 to 99.73%), precision (2.84 to 8.02%) and spiked recovery (&gt;95%) are within the FDA guidelines. This method is highly sensitive [limit of detection (LOD) of 1\u2009ng\/mL] with a dynamic range of 3.12 to 200\u2009ng\/mL. The endogenous FA level in pasteurized cow milk is 70\u2009ng\/mL (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200960). The FA content in raw milk samples from cow, goat and buffalo (each\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910) varied from 134 to 255\u2009ng\/mL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This method is precise and sufficiently sensitive to quantify endogenous FA in milk samples using a minimal sample volume. As it involves simple sample preparation steps, it can be used routinely to quantify endogenous FA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8865","type":"journal-article","created":{"date-parts":[[2020,6,19]],"date-time":"2020-06-19T12:26:23Z","timestamp":1592569583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Simple click chemistry\u2010based derivatization to quantify endogenous formaldehyde in milk using ultra\u2010high\u2010performance liquid chromatography\/tandem mass spectrometry in selected reaction monitoring mode"],"prefix":"10.1002","volume":"34","author":[{"given":"Sweekruthi A.","family":"Shetty","sequence":"first","affiliation":[{"name":"Food Safety and Analytical Quality Control Laboratory CSIR \u2013 Central Food Technological Research Institute  Cheluvamba Mansion Mysore 570020 India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0037-4729","authenticated-orcid":false,"given":"Kannan","family":"Rangiah","sequence":"additional","affiliation":[{"name":"Food Safety and Analytical Quality Control Laboratory CSIR \u2013 Central Food Technological Research Institute  Cheluvamba Mansion Mysore 570020 India"},{"name":"Institute of Bioinformatics  Discoverer Building, 7th Floor, International Tech Park Whitefield Bangalore Karnataka 560066 India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"National Research Council (US) Committee on Toxicology.Formaldehyde \u2013 An assessment of its health effects. Washington (DC): National Academies Press (US).1980. Effects on humans. Available from:https:\/\/www.ncbi.nlm.nih.gov\/books\/NBK217652\/"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(09)70358-4"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.15171\/hpp.2015.035"},{"key":"e_1_2_8_5_1","volume-title":"Monographs on the Evaluation of Carcinogenic Risks to Humans","author":"International Agency for Research on Cancer","year":"2004"},{"key":"e_1_2_8_6_1","unstructured":"Merchant Research and Consulting Ltd.Formaldehyde: 2016 world market outlook and forecast up to 2020. Available:http:\/\/mcgroup.co.uk\/researches\/formaldehyde. 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