{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T10:33:10Z","timestamp":1777545190664,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,9,15]],"date-time":"2020-09-15T00:00:00Z","timestamp":1600128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2020,11,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Glycosyl\u2010inositol\u2010phospho\u2010ceramides (GIPCs) or glycosylphosphatidylinositol\u2010anchored fungal polysaccharides are known to be major lipids in plant and fungal plasma membranes and to play an important role in stress adaption. However, their analysis remains challenging due to the several steps involved for their extractions and purifications prior to mass spectrometric analysis. To address this challenge, we developed a rapid and sensitive method to identify GIPCs from the four common fungal plant pathogens\n                      <jats:italic>Botrytis cinerea<\/jats:italic>\n                      ,\n                      <jats:italic>Fusarium graminearium<\/jats:italic>\n                      ,\n                      <jats:italic>Neurospora crassa<\/jats:italic>\n                      and\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Ustilago maydis<\/jats:italic>\n                      <\/jats:styled-content>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Fungal plant pathogens were cultured, harvested, heat\u2010inactivated and washed three times with double\u2010distilled water. Intact fungi were deposited on a matrix\u2010assisted laser desorption ionization (MALDI) target plate, mixed with the matrix consisting of a 9:1 mixture of 2,5\u2010dihydroxybenzoic acid and 2\u2010hydroxy\u20105\u2010methoxybenzoic acid solubilized at 10\u2009mg\/mL in chloroform\u2013methanol (9:1 v\/v) and analyzed using a Bruker MALDI Biotyper Sirius system in the linear negative ion mode. Mass spectra were acquired from\n                      <jats:italic>m<\/jats:italic>\n                      \/\n                      <jats:italic>z<\/jats:italic>\n                      700 to 2000.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      MALDI time\u2010of\u2010flight (TOF) mass spectrometric analysis of cultured fungi showed clear signature of GIPCs in\n                      <jats:italic>B. cinerea<\/jats:italic>\n                      ,\n                      <jats:italic>F. graminearium<\/jats:italic>\n                      ,\n                      <jats:italic>N. crassa<\/jats:italic>\n                      and\n                      <jats:italic>\n                        <jats:styled-content style=\"fixed-case\">U. maydis<\/jats:styled-content>\n                        .\n                      <\/jats:italic>\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We have demonstrated that routine MALDI\u2010TOF in the linear negative ion mode combined with an apolar solvent system to solubilize the matrix is applicable to the detection of filamentous fungal GIPCs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8904","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T21:09:39Z","timestamp":1595452179000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Rapid glycosyl\u2010inositol\u2010phospho\u2010ceramide fingerprint from filamentous fungal pathogens using the MALDI Biotyper Sirius system"],"prefix":"10.1002","volume":"34","author":[{"given":"Kofo","family":"Saromi","sequence":"first","affiliation":[{"name":"Department of Chemistry Faculty of Natural Sciences and Institute of Chemical Biology (ICB), Imperial College London  South Kensington Campus London SW7 2AZ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippa","family":"England","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences Imperial College London  London SW7 2AZ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenhao","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Mathematics Imperial College London  London SW7 2AZ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Kostrzewa","sequence":"additional","affiliation":[{"name":"Bruker Daltonik GmbH  Bremen 28359 Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy","family":"Corran","sequence":"additional","affiliation":[{"name":"Syngenta Group, Bioscience, Jealott's Hill Research Station, Bracknell RG42 6EY  UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rudiger","family":"Woscholski","sequence":"additional","affiliation":[{"name":"Department of Chemistry Faculty of Natural Sciences and Institute of Chemical Biology (ICB), Imperial College London  South Kensington Campus London SW7 2AZ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6614-8698","authenticated-orcid":false,"given":"Gerald","family":"Larrouy\u2010Maumus","sequence":"additional","affiliation":[{"name":"MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences Imperial College London  London SW7 2AZ UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,15]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"9724283","article-title":"Plant fungal pathogenesis","volume":"2017","author":"Yang J","year":"2017","journal-title":"Biomed Res Int"},{"issue":"1","key":"e_1_2_6_3_1","first-page":"1","article-title":"Plant pathogenic fungi","volume":"5","author":"Doehlemann G","year":"2017","journal-title":"J Microbiol Spectr"},{"issue":"7","key":"e_1_2_6_4_1","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S1360-1385(03)00128-6","article-title":"Sphingolipids, new players in plant signaling","volume":"8","author":"Worrall D","year":"2003","journal-title":"Trends Plant Sci"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2016.1152438"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00138"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-5822.2011.01735.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0001-37652004000300010"},{"issue":"1","key":"e_1_2_6_9_1","doi-asserted-by":"crossref","first-page":"e1003832","DOI":"10.1371\/journal.ppat.1003832","article-title":"Synthesis and biological properties of fungal glucosylceramide","volume":"10","author":"Poeta M","year":"2014","journal-title":"PLoS Pathog"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3321"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00642-18"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M604050200"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-013-7130-8"},{"issue":"1","key":"e_1_2_6_14_1","doi-asserted-by":"crossref","first-page":"19","DOI":"10.3390\/jof4010019","article-title":"Glycosylphosphatidylinositol anchors from galactomannan and GPI\u2010anchored protein are synthesized by distinct pathways in Aspergillus fumigatus","volume":"4","author":"Li J","year":"2018","journal-title":"J Fungi (Basel)"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/EC.00109-08"},{"issue":"26","key":"e_1_2_6_16_1","doi-asserted-by":"crossref","first-page":"12147","DOI":"10.1016\/S0021-9258(18)67215-1","article-title":"Identification of a glycolipid precursor of the Trypanosoma brucei variant surface glycoprotein","volume":"261","author":"Krakow JL","year":"1986","journal-title":"J Biol Chem"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.71.110601.135414"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.D500014-JLR200"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carres.2003.07.010"},{"issue":"3","key":"e_1_2_6_20_1","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1042\/bst0090191","article-title":"Bacterial lipopolysaccharide and its lipid A component: Some historical and some current aspects","volume":"9","author":"Westphal O","year":"1981","journal-title":"Biochem Soc Trans"},{"issue":"21","key":"e_1_2_6_21_1","first-page":"17","article-title":"Chemical and biological analysis of highly purified bacterial polysaccharides","volume":"78","author":"Westphal O","year":"1953","journal-title":"Dtsch Med Wochenschr"},{"key":"e_1_2_6_22_1","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1016\/S0076-6879(10)80005-9","article-title":"Microbe\u2010associated molecular patterns in innate immunity: Extraction and chemical analysis of Gram\u2010negative bacterial lipopolysaccharides","volume":"480","author":"Castro C","year":"2010","journal-title":"Methods Enzymol"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1994"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.8b02611"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.9b03340"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-04793-4"},{"issue":"8","key":"e_1_2_6_27_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/femspd\/ftw097","article-title":"Norharmane matrix enhances detection of endotoxin by MALDI\u2010MS for simultaneous profiling of pathogen, host, and vector systems","volume":"74","author":"Scott AJ","year":"2016","journal-title":"Pathog Dis"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2015.12.004"},{"issue":"1","key":"e_1_2_6_29_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.tube.2014.11.001","article-title":"Mycobacterial envelope lipids fingerprint from direct MALDI\u2010TOF MS analysis of intact bacilli","volume":"95","author":"Larrouy\u2010Maumus G","year":"2016","journal-title":"Tuberculosis (Edinb)"},{"issue":"12","key":"e_1_2_6_30_1","doi-asserted-by":"crossref","first-page":"e01427\u201019","DOI":"10.1128\/JCM.01427-19","article-title":"Detection of colistin resistance in Escherichia coli using the MALDI Biotyper Sirius mass spectrometry system","volume":"57","author":"Furniss RCD","year":"2019","journal-title":"J Clin Microbiol"},{"issue":"12","key":"e_1_2_6_31_1","first-page":"3359","article-title":"Rapid detection and discrimination of chromosome\u2010 and MCR\u2010plasmid\u2010mediated resistance to polymyxins by MALDI\u2010TOF MS in Escherichia coli: The MALDIxin test","volume":"73","author":"Dortet L","year":"2018","journal-title":"J Antimicrob Chemother"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2017.08.008"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1008331"},{"key":"e_1_2_6_34_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0218951"},{"key":"e_1_2_6_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts447"},{"key":"e_1_2_6_36_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsta.2015.0371"},{"key":"e_1_2_6_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr010375i"},{"key":"e_1_2_6_38_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-006-0090-6"},{"key":"e_1_2_6_39_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M510163200"},{"key":"e_1_2_6_40_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.398321"},{"key":"e_1_2_6_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwv059"},{"key":"e_1_2_6_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm122"},{"key":"e_1_2_6_43_1","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M700149-JLR200"},{"key":"e_1_2_6_44_1","first-page":"213","article-title":"Analysis of the carbohydrate and lipid components of glycosylphosphatidylinositol structures","volume":"76","author":"Treumann A","year":"1998","journal-title":"Methods Mol Biol"},{"issue":"3","key":"e_1_2_6_45_1","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1093\/glycob\/cwg004","article-title":"Structures of the glycosylphosphatidylinositol membrane anchors from Aspergillus fumigatus membrane proteins","volume":"13","author":"Fontaine T","year":"2003","journal-title":"Glycobiology"},{"key":"e_1_2_6_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(02)00337-2"},{"key":"e_1_2_6_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2006.03.004"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.8904","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8904","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.8904","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.8904","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T10:14:14Z","timestamp":1761473654000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.8904"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,15]]},"references-count":46,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2020,11,30]]}},"alternative-id":["10.1002\/rcm.8904"],"URL":"https:\/\/doi.org\/10.1002\/rcm.8904","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.8904\/v3\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v2\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v1\/review3","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.8904\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,9,15]]},"assertion":[{"value":"2020-04-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-15","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e8904"}}