{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:14:59Z","timestamp":1772118899142,"version":"3.50.1"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T00:00:00Z","timestamp":1604448000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000265","name":"Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2021,1,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Matrix\u2010assisted laser desorption\/ionization mass spectrometry imaging (MALDI\u2010MSI) is routinely employed to monitor the distribution of compounds in tissue sections and generate two\u2010dimensional (2D) images. Whilst informative the images do not represent the distribution of the analyte of interest through the entire organ. The generation of 3D images is an exciting field that can provide a deeper view of the analyte of interest throughout an entire organ.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Serial sections of mouse and rat lung tissue were obtained at 120\u2009\u03bcm depth intervals and imaged individually. Homogenate registration markers were incorporated in order to aid the final 3D image construction. Using freely available software packages, the images were stacked together to generate a 3D image that showed the distribution of endogenous species throughout the lungs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Preliminary tests were performed on 16 serial tissue sections of mouse lungs. A 3D model showing the distribution of phosphocholine at\n                      <jats:italic>m\/z<\/jats:italic>\n                      184.09 was constructed, which defined the external structure of the lungs and trachea. Later, a second experiment was performed using 24 serial tissue sections of the left lung of a rat. Two molecular markers, identified as [PC (32:1)\u2009+\u2009K]\n                      <jats:sup>+<\/jats:sup>\n                      at\n                      <jats:italic>m\/z<\/jats:italic>\n                      770.51 and [PC (36:4)\u2009+\u2009K]\n                      <jats:sup>+<\/jats:sup>\n                      at\n                      <jats:italic>m\/z<\/jats:italic>\n                      820.52, were used to generate 3D models of the parenchyma and airways, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A straightforward method to generate 3D MALDI\u2010MS images of selected molecules in lung tissue has been presented. Using freely available imaging software, the 3D distributions of molecules related to different anatomical features were determined.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.8957","type":"journal-article","created":{"date-parts":[[2020,9,29]],"date-time":"2020-09-29T09:04:35Z","timestamp":1601370275000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Monitoring the three\u2010dimensional distribution of endogenous species in the lungs by matrix\u2010assisted laser desorption\/ionization mass spectrometry imaging"],"prefix":"10.1002","volume":"35","author":[{"given":"Bryn","family":"Flinders","sequence":"first","affiliation":[{"name":"Centre for Mass Spectrometry Imaging, Biomedical Research Centre, City Campus Sheffield Hallam University  Sheffield S1 1WB UK"},{"name":"Hair Diagnostix Dutch Screening Group  Gaetano Martinolaan 63A Maastricht 6229 GS The Netherlands"},{"name":"Maastricht Multimodal Molecular Imaging Institute (M4I), Maastricht University, Universiteitssingel 50  Maastricht 6229 ER The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josie","family":"Morrell","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline  Gunnels Wood Road Stevenage SG1 2NY UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter S.","family":"Marshall","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline  Gunnels Wood Road Stevenage SG1 2NY UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa E.","family":"Ranshaw","sequence":"additional","affiliation":[{"name":"GlaxoSmithKline  Gunnels Wood Road Stevenage SG1 2NY UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6533-7179","authenticated-orcid":false,"given":"Ron M.A.","family":"Heeren","sequence":"additional","affiliation":[{"name":"Maastricht Multimodal Molecular Imaging Institute (M4I), 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