{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:34:58Z","timestamp":1791038098949,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2021,9,29]],"date-time":"2021-09-29T00:00:00Z","timestamp":1632873600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2021,11,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Digoxin is widely used in the clinical treatment of cardiovascular diseases. However, due to its extremely narrow therapeutic window, therapeutic drug monitoring (TDM) is vitally important. In consideration of the time\u2010consuming and labor\u2010intensive nature of the traditional techniques, an automated and efficient method was required for the clinical individualized TDM of digoxin.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      An online solid\u2010phase extraction liquid chromatography tandem high\u2010resolution mass spectrometry (online\u2010SPE\u2010LC\u2010HRMS) method was developed and applied for the determination of digoxin in plasma. The online SPE\u2010LC steps included pretreatment and separation of plasma samples that were carried out using a Waters Oasis HLB cartridge and XBridge Shield RP18 column, respectively. A high\u2010resolution Q Orbitrap mass spectrometer with targeted\u2010selected ion monitoring in negative scan mode was applied to monitor formate\u2010adduct ions [M\u2009+\u2009HCOO]\n                      <jats:sup>\u2212<\/jats:sup>\n                      <jats:italic>m\/z<\/jats:italic>\n                      825.42781 for digoxin.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Linearity was shown over the range 0.1\u201310\u00a0ng\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      for digoxin with correlation coefficients of R\n                      <jats:sup>2<\/jats:sup>\n                      \u2009&gt;\u20090.999. The lower limit of quantitation (LLOQ) for digoxin was 0.1\u00a0ng\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      . Extraction recoveries ranged from 82.61% to 94.28% for digoxin. The intra\u2010 and inter\u2010day precision values were &lt; 5.53% with accuracy ranging from 84.97% to 96.75%. The total running time was 10\u00a0min for each sample.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The established method displayed satisfactory recoveries, accuracy, precision, and stability, and successfully applied on the TDM of digoxin. This automated streamlined method provides a powerful tool to guide the individualized administration of digoxin, which is significant for the practice of precision medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9191","type":"journal-article","created":{"date-parts":[[2021,9,7]],"date-time":"2021-09-07T01:49:54Z","timestamp":1630979394000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["An automated streamlined method for the therapeutic drug monitoring of digoxin based on the online\u2010solid\u2010phase extraction liquid chromatography tandem high\u2010resolution mass spectrometry"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8871-3632","authenticated-orcid":false,"given":"Congmin","family":"Liu","sequence":"first","affiliation":[{"name":"College of Chemistry and Pharmaceutical Sciences Qingdao Agricultural University  Qingdao 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