{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:06Z","timestamp":1772118906055,"version":"3.50.1"},"reference-count":18,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2021,12,1]],"date-time":"2021-12-01T00:00:00Z","timestamp":1638316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007820","name":"Hangzhou Normal University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007820","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2022,1,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Stereoisomer profiling is always a difficult issue. Based on the difference between diastereomers, usually because of steric hindrance, isomers can be differentiated by mass spectrometry (MS), although it is often not an easy task. In the current study, tetracycline, chlortetracycline and doxycycline could be distinguished from their respective 4\u2010epimers by MS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The electrospray ionization tandem mass spectrometry (ESI\u2010MS\n                      <jats:sup>n<\/jats:sup>\n                      ) analyses were carried out on a Bruker 3000\n                      <jats:sup>plus<\/jats:sup>\n                      ion trap mass spectrometer. For MS\/MS experiments, the collision energy was set between 0.18 and 0.45\u2009V to perform energy\u2010resolved mass spectrometry (ERMS). Test solutions were prepared in methanol\/water (90:10, v\/v) at a concentration of 10\u00a0\u03bcg\/mL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared with the collision\u2010induced dissociation (CID) spectrum of protonated tetracycline, the most abundant peak changed from\n                      <jats:italic>m\/z<\/jats:italic>\n                      427 to\n                      <jats:italic>m\/z<\/jats:italic>\n                      410 for 4\u2010epitetracycline. For chlortetracycline and its 4\u2010epimer, differences in relative abundance were observed too. In the CID spectrum of a fragment ion of doxycycline, the abundance of\n                      <jats:italic>m\/z<\/jats:italic>\n                      154 was relatively higher than for the 4\u2010epimer, showing the same trend as in the CID spectra of the other two pairs of tetracyclines.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The CID spectra of tetracycline and chlortetracycline were different from those of their 4\u2010epimers. The CID spectra of protonated doxycycline and its 4\u2010epimer showed only a subtle difference, but the\n                      <jats:italic>m\/z<\/jats:italic>\n                      154 fragment ion in the CID spectra of the fragment ion at\n                      <jats:italic>m\/z<\/jats:italic>\n                      428 offers the possibility to differentiate both epimers.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9221","type":"journal-article","created":{"date-parts":[[2021,11,10]],"date-time":"2021-11-10T20:58:15Z","timestamp":1636577895000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Diastereomer recognition of three pairs of tetracyclines by electrospray ionization mass spectrometry"],"prefix":"10.1002","volume":"36","author":[{"given":"Peixi","family":"Zhu","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Sciences Zhejiang University of Technology  Zhejiang Hangzhou China"},{"name":"Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven University of Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luxi","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences Zhejiang University of Technology  Zhejiang Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9908-7772","authenticated-orcid":false,"given":"Kezhi","family":"Jiang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organosilicon Chemistry and Material Technology Hangzhou Normal University  Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weike","family":"Su","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Sciences Zhejiang University of Technology  Zhejiang Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ann","family":"Van Schepdael","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven University of Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4545-2989","authenticated-orcid":false,"given":"Erwin","family":"Adams","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analysis, Department of Pharmaceutical and Pharmacological Sciences, KU Leuven University of Leuven  Leuven Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,12]]},"reference":[{"key":"e_1_2_3_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2018.04.093"},{"key":"e_1_2_3_3_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b03735"},{"key":"e_1_2_3_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.3939"},{"key":"e_1_2_3_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b03804"},{"key":"e_1_2_3_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2021.114125"},{"key":"e_1_2_3_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.8421"},{"key":"e_1_2_3_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpca.0c11540"},{"key":"e_1_2_3_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2020.122457"},{"key":"e_1_2_3_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-2670(03)00341-6"},{"key":"e_1_2_3_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2095-3119(12)60114-2"},{"key":"e_1_2_3_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.2991"},{"key":"e_1_2_3_13_1","doi-asserted-by":"publisher","DOI":"10.13005\/ojc\/330539"},{"key":"e_1_2_3_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2019.121807"},{"key":"e_1_2_3_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/19440049.2018.1427894"},{"key":"e_1_2_3_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.7252"},{"key":"e_1_2_3_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4476"},{"key":"e_1_2_3_18_1","volume-title":"Gaussian 03","author":"Frisch MJ","year":"2003"},{"key":"e_1_2_3_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-0305(02)00356-2"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9221","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/rcm.9221","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9221","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T11:21:38Z","timestamp":1761477698000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.9221"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,12]]},"references-count":18,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,1,30]]}},"alternative-id":["10.1002\/rcm.9221"],"URL":"https:\/\/doi.org\/10.1002\/rcm.9221","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.9221\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9221\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9221\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9221\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,12]]},"assertion":[{"value":"2021-09-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-11-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-12-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e9221"}}