{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:14:11Z","timestamp":1772118851940,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2022,4,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Foscarnet (FCV) is used to treat cytomegalovirus, human herpesvirus, and human immunodeficiency virus infections. It is a low\u2010molecular\u2010weight compound containing carboxylate and phosphate groups. There are no reports on the use of liquid chromatography\u2013tandem mass spectrometry (LC\/MS\/MS) to analyze FCV via bioanalysis. In the present study, we developed the ion\u2010pair LC\/MS\/MS method to analyze FCV in human serum and cerebrospinal fluid (CSF).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      FCV was extracted from human serum and CSF by weak anion exchange (WAX) solid\u2010phase extraction. The LC\/MS\/MS systems were coated with 0.1% phosphoric acid in methanol to avoid nonspecific absorption. FCV was detected using ion\u2010pair LC\/MS\/MS with dibutylammonium acetate. Selected reaction monitoring transition of FCV in the negative ion mode was selected at\n                      <jats:italic>m<\/jats:italic>\n                      \/\n                      <jats:italic>z<\/jats:italic>\n                      125.1\u2009\u2192\u200962.9.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FCV was selectively detected in human serum and CSF, and the liner range was 5\u20131000\u2009\u03bcM (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.99). The intraday and interday accuracy and precision were within \u00b115%. FCV was constantly extracted from human serum and CSF by WAX solid\u2010phase extraction (recovery ratio\u2009=\u200976.0\u201377.9%). No matrix effect was observed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>A robust LC\/MS\/MS method to analyze FCV was successfully developed. FCV was selectively measured using LC\/MS\/MS in very low extract volumes (20\u2009\u03bcL). The method will be useful in evaluating the FCV level in human serum and CSF.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9255","type":"journal-article","created":{"date-parts":[[2022,1,10]],"date-time":"2022-01-10T03:48:37Z","timestamp":1641786517000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Use of liquid chromatography\u2013tandem mass spectrometry for foscarnet quantification in human serum and cerebrospinal fluid"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0820-1208","authenticated-orcid":false,"given":"Kei","family":"Irie","sequence":"first","affiliation":[{"name":"Faculty of Pharmaceutical Science Kobe Gakuin University  Kobe Japan"},{"name":"Department of Pharmacy Kobe City Hospital Organization, Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nobuhiro","family":"Hiramoto","sequence":"additional","affiliation":[{"name":"Department of Hematology Kobe City Hospital Organization, Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayuki","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Hematology Kobe City Hospital Organization, Kobe City Medical Center General Hospital  Kobe Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shoji","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Faculty of Pharmaceutical Science Kobe Gakuin University  Kobe 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