{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:15Z","timestamp":1772118735879,"version":"3.50.1"},"reference-count":23,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2022,6,17]],"date-time":"2022-06-17T00:00:00Z","timestamp":1655424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000066","name":"National Institute of Environmental Health Sciences","doi-asserted-by":"publisher","award":["P42ES017198"],"award-info":[{"award-number":["P42ES017198"]}],"id":[{"id":"10.13039\/100000066","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2022,8,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      We studied an aldehyde\u2010labeling reagent,\n                      <jats:italic>N<\/jats:italic>\n                      \u2010{2\u2010[(4\u2010aminophenoxy)methyl]benzyl}\u2010\n                      <jats:italic>N,N<\/jats:italic>\n                      \u2010diethylethanaminium bromide (CAX\u2010A), containing an aniline functional group for the detection of aldehydes with high specificity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Six standard aldehydes were labeled by CAX\u2010A and analyzed using LC\u2010ESI\u2010Orbitrap\u2010MS. The aldehydes (each 40\u2009nmol) were derivatized with CAX\u2010A in the presence of sodium cyanoborohydride at room temperature overnight. The labeling reaction was applied to two urine samples for the detection of putative aldehydes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>All six standard CAX\u2010aldehyde derivatives were detected as precursor ions by dilution to 830\u2009fmol\/injection (signal\u2010to\u2010noise [S\/N] ratio 587\u20131573). A total of 2184 MS1 features were detected overall in urine and blanks, of which 14 were putative aldehydes found only in urine.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>CAX\u2010A can provide three levels of specificity for aldehyde detection. First is the known labeling specificity of the aniline functional group for aldehydes, which we confirmed here by observing a significant peak only from the aldehyde (S\/N\u2009=\u20093388) when a mixture of an aldehyde, a ketone (no peak), and a quinone (S\/N\u2009=\u20092.3) was tested. Second is the ease of formation of an analyte\u2010characteristic first product ion (via anchimeric\u2010assisted loss of triethylamine as a neutral) in MS2 from a CAX\u2010labeled analyte. Third is the formation of a characteristic second product ion via loss of CO in MS3. CAX\u2010A enables the specific, convenient detection of putative aldehydes in urine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9322","type":"journal-article","created":{"date-parts":[[2022,5,4]],"date-time":"2022-05-04T03:08:46Z","timestamp":1651633726000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A high\u2010specificity aniline\u2010based mass tag for aldehyde detection"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3360-5914","authenticated-orcid":false,"given":"Prisca","family":"Mungalachetty","sequence":"first","affiliation":[{"name":"Bouv\u00e9 College of Health Sciences, Department of Pharmaceutical Science Northeastern University  Boston MA 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