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Given the importance of surface viral proteins for interactions with healthy tissue, they are targets of interest for mass spectrometry\u2010based analysis. For that reason, the possibility of detecting and quantifying peptides using a high\u2010throughput technique, laser diode thermal desorption\u2013triple quadrupole mass spectrometry (LDTD\u2010QqQMS), was explored.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Two peptides used as models for small peptides (leu\u2010enkephalin and endomorphin\u20102) and four tryptic peptides (GVYYPDK, NIDGYFK, IADYNYK, and QIAPGQTGK) specific to the SARS\u2010CoV\u20102 Spike protein were employed. Target peptides were analyzed individually in the positive mode by LDTD\u2010QqQMS. Peptides were quantified by internal calibration using selected reaction monitoring transitions in pure solvents and in samples spiked with 20\u2009\u03bcg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      of a bovine serum albumin tryptic digest to represent real analysis conditions.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Low\u2010energy fragment ions (\n                      <jats:italic>b<\/jats:italic>\n                      and\n                      <jats:italic>y<\/jats:italic>\n                      ions) as well as high\u2010energy fragment ions (\n                      <jats:italic>c<\/jats:italic>\n                      and\n                      <jats:italic>x<\/jats:italic>\n                      ions) and some of their corresponding water or ammonia losses were detected in the full mass spectra. Only for the smallest peptides, leu\u2010enkephalin and endomorphin\u20102, were [M\u2009+\u2009H]\n                      <jats:sup>+<\/jats:sup>\n                      ions observed. Product ion spectra confirmed that, with the experimental conditions used in the present study, LDTD transfers a considerable amount of energy to the target peptides. Quantitative analysis showed that it was possible to quantify peptides using LDTD\u2010QqQMS with acceptable calibration curve linearity (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009&gt;\u20090.99), precision (RSD\u2009&lt;\u200918.2%), and trueness (bias\u2009&lt;\u20098.3%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study demonstrated for the first time that linear peptides can be qualitatively and quantitatively analyzed using LDTD\u2010QqQMS. Limits of quantification and dynamic ranges are still inadequate for clinical applications, but other applications where higher levels of proteins must be detected could be possible with LDTD. Given the high\u2010throughput capabilities of LDTD\u2010QqQMS (&gt;15\u2009000 samples in less than 43\u2009h), more studies are needed to improve the sensitivity for peptide analysis of this technique.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9373","type":"journal-article","created":{"date-parts":[[2022,8,7]],"date-time":"2022-08-07T02:39:46Z","timestamp":1659839986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Ultrafast analysis of peptides by laser diode thermal desorption\u2013triple quadrupole mass spectrometry"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4552-4903","authenticated-orcid":false,"given":"Pedro A.","family":"Segura","sequence":"first","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9dric","family":"Guillaumain","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emmanuel","family":"Eysseric","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judith","family":"Boudrias","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00e9gane","family":"Moreau","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cassandra","family":"Gu\u00e9rette","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R\u00e9mi","family":"Cl\u00e9mencin","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Faculty of Sciences Universit\u00e9 de Sherbrooke  Sherbrooke QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-7333","authenticated-orcid":false,"given":"Francis","family":"Beaudry","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Biom\u00e9decine V\u00e9t\u00e9rinaire, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire Universit\u00e9 de Montr\u00e9al  Saint\u2010Hyacinthe QC Canada"},{"name":"Centre de recherche sur le cerveau et l'apprentissage (CIRCA) Universit\u00e9 de Montr\u00e9al  Montr\u00e9al QC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102008\u20103"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010020\u201002661\u20102"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/d41586\u2010021\u201000332\u20104"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMcp2117115"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010020\u20102180\u20105"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb2762"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.701501"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12929\u2010021\u201000784\u2010w"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/Spectrum.00247\u201021"},{"key":"e_1_2_7_11_1","unstructured":"BBC News.Covid PCR tests end for asymptomatic cases in England. 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