{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:15Z","timestamp":1772118735929,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2022,9,16]],"date-time":"2022-09-16T00:00:00Z","timestamp":1663286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["42177330"],"award-info":[{"award-number":["42177330"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41771334"],"award-info":[{"award-number":["41771334"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["41977100"],"award-info":[{"award-number":["41977100"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019081","name":"Science and Technology Program of Hunan Province","doi-asserted-by":"publisher","award":["2020JJ4655"],"award-info":[{"award-number":["2020JJ4655"]}],"id":[{"id":"10.13039\/501100019081","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2022,11,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>The amino acid\u2013nitrogen (AA\u2010N) isotope analysis of naturally abundant or isotope\u2010labeled samples is indispensable for tracing nitrogen transfer in soil nitrogen biogeochemical cycling processes. Despite the usefulness of AA\u2010N isotope analysis, the preparation methods are complex and time\u2010consuming, and necessitate the use of toxic reagents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We present an improved, rapid method for AA\u2010N isotope analysis with high precision. At a high pH, AA\u2010N was released and oxidized to N\n                      <jats:sub>2<\/jats:sub>\n                      O using ClO\n                      <jats:sup>\u2212<\/jats:sup>\n                      under vacuum. Additionally, purge\u2010and\u2010trap isotope ratio mass spectrometry was used to analyze N\n                      <jats:sub>2<\/jats:sub>\n                      O. Moreover, we investigated the effect of various factors on the N\n                      <jats:sub>2<\/jats:sub>\n                      O conversion process with glycine and applied the results to seven representative single\u2010N AAs (alanine, serine, cysteine, aspartic acid, glutamic acid, leucine, and phenylalanine) and five poly\u2010N AAs (lysine, arginine, histidine, tryptophan, and asparagine), as well as side\u2010chain analogs, blank reagent, and other N forms.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The concentration of ClO\n                      <jats:sup>\u2212<\/jats:sup>\n                      and the pH were determined to be crucial factors for achieving desirable AA\u2010N to N\n                      <jats:sub>2<\/jats:sub>\n                      O conversion efficiencies. Glycine\u2010N had the highest N\n                      <jats:sub>2<\/jats:sub>\n                      O yield of 70%, with isotopic results consistent with those of the reference values at a high precision (within 0.5\u2030 for natural abundance and 0.01 atom% for\n                      <jats:sup>15<\/jats:sup>\n                      N\u2010enrichment) at the nanomolar N level. Additionally, the \u03b1\u2010NH\n                      <jats:sub>2<\/jats:sub>\n                      AAs were labile, and the single\u2010N AAs were more easily converted to N\n                      <jats:sub>2<\/jats:sub>\n                      O than poly\u2010N AAs. With the exception of \u03b3\u2010aminobutyric acid, the N\n                      <jats:sub>2<\/jats:sub>\n                      O conversion efficiencies of the side\u2010chain N analogs were very low (below 5%). This method was also applicable to the\n                      <jats:sup>15<\/jats:sup>\n                      N analysis of the total free AAs in complex soil samples without interference from analytical blanks and other forms of N.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Our method is highly selective for the \u03b1\u2010NH\n                      <jats:sub>2<\/jats:sub>\n                      groups of an amino acid, and the oxidation of the side chain is difficult. In addition, the method is sensitive, rapid, and convenient, and does not require toxic reagents.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9390","type":"journal-article","created":{"date-parts":[[2022,9,2]],"date-time":"2022-09-02T23:49:36Z","timestamp":1662162576000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Rapid, sensitive analysis method for determining the nitrogen stable isotope ratio of total free amino acids in soil"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8687-1672","authenticated-orcid":false,"given":"Hongzhao","family":"Yuan","sequence":"first","affiliation":[{"name":"Key Laboratory of Agro\u2010ecological Processes in Subtropical Region Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"He","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro\u2010ecological Processes in Subtropical Region Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiangbi","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro\u2010ecological Processes in Subtropical Region Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tida","family":"Ge","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro\u2010products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province Institute of Plant Virology, Ningbo University  Ningbo Zhejiang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro\u2010ecological Processes in Subtropical Region Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling  Changsha Hunan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiurong","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Agro\u2010ecological Processes in Subtropical Region Institute of Subtropical Agriculture, Chinese Academy of Sciences, Mapoling  Changsha Hunan 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