{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:12:39Z","timestamp":1772118759526,"version":"3.50.1"},"reference-count":56,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,1,11]],"date-time":"2023-01-11T00:00:00Z","timestamp":1673395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["EXC2186\u201cTheFuelScienceCenter\u201dID390919832"],"award-info":[{"award-number":["EXC2186\u201cTheFuelScienceCenter\u201dID390919832"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2023,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Methyl ketones are of interest for the application as biofuels. The fatty acid metabolism of different microbes has been rearranged to achieve a sustainable production of methyl ketones. The biofuel properties and possible further chemical modifications of these methyl ketones are influenced by their chain length, as well as their degree of saturation and the corresponding double bond position.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A method based on gas chromatography\u2013electron ionization; mass spectrometry (GC\u2010EI\u2010MS) was used to determine the double bond position of methyl ketones. Derivatization using dimethyl disulfide (DMDS) and an iodine catalyst enabled the activation of the double bonds for selective fragmentation during electron ionization. The cleavage led to key fragments in the Orbitrap high\u2010resolution mass spectrum and allowed the unequivocal localization of the double bond position of the respective monounsaturated methyl ketone.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The double bond position of several medium chain length methyl ketones originating from the gram\u2010negative bacterium\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>Pseudomonas taiwanensis<\/jats:italic>\n                      <\/jats:styled-content>\n                      (\n                      <jats:italic>P<\/jats:italic>\n                      .\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>taiwanensis)<\/jats:italic>\n                      <\/jats:styled-content>\n                      VLB120 was determined. The DMDS derivatives of methyl ketones can yield isobaric fragment ions for different possible double bond positions, which can be distinguished only using high\u2010resolution MS. The double bond position of all methyl ketones deriving from\n                      <jats:styled-content style=\"fixed-case\">\n                        <jats:italic>P. taiwanensis<\/jats:italic>\n                      <\/jats:styled-content>\n                      VLB120 was the same, counting from the end of the aliphatic chain, and was determined as \u03c9\u20107.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The derivatization of medium chain length monounsaturated methyl ketones with DMDS allowed the determination of the corresponding double bond position via GC\u2010EI\u2010MS. High\u2010resolution MS is needed to differentiate possible double bond positions that yield isobaric fragment ions.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9457","type":"journal-article","created":{"date-parts":[[2022,12,13]],"date-time":"2022-12-13T12:43:45Z","timestamp":1670935425000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Determination of double bond positions in methyl ketones by gas chromatography\u2013mass spectrometry using dimethyl disulfide derivatives"],"prefix":"10.1002","volume":"37","author":[{"given":"Matti","family":"Froning","sequence":"first","affiliation":[{"name":"Institute of Inorganic and Analytical Chemistry, University of M\u00fcnster  M\u00fcnster Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolin","family":"Gr\u00fctering","sequence":"additional","affiliation":[{"name":"Institute of Applied Microbiology, Aachen Biology and 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