{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:41:25Z","timestamp":1790271685123,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2003,2,18]],"date-time":"2003-02-18T00:00:00Z","timestamp":1045526400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2003,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ion\u2010pair reversed\u2010phase high\u2010performance liquid chromatography (IP\u2010RP\u2010HPLC) was utilized in tandem with negative\u2010ion electrospray ionization time\u2010of\u2010flight mass spectrometry (ESI\u2010TOFMS) for the analysis of native and chemically modified oligonucleotides. Separation was performed on a 1.0\u2009\u00d7\u200950\u2009mm column packed with porous C\n                    <jats:sub>18<\/jats:sub>\n                    sorbent with a particle size of 2.5\u2009\u03bcm and an average pore diameter of 140 \u00c5. A method was developed which maximizes both chromatographic separation and mass spectrometric sensitivity using an optimized buffer system containing triethylamine and 1,1,1,3,3,3\u2010hexafluoro\u20102\u2010propanol with a methanol gradient. The ESI\u2010TOFMS tuning parameters were also optimized in order to minimize in\u2010source fragmentation and achieve the best sensitivity. Analyses of native, phosphorothioate, and guanine\u2010rich oligonucleotides were performed by LC\/MS. Detection limits were at sub\u2010picomole levels with an average mass accuracy of 125\u2009ppm. The described method allowed for the LC\/MS analysis of oligonucleotides up to 110mer in length with little alkali cation adduction. Since sensitive detection of oligonucleotides was achieved with ultraviolet (UV) detection, we utilized a combination of UV\u2010MS for quantitation (UV) and characterization (MS) of oligonucleotides and their failure sequence fragments\/metabolites. Copyright \u00a9 2003 John Wiley &amp; Sons, Ltd.\n                  <\/jats:p>","DOI":"10.1002\/rcm.959","type":"journal-article","created":{"date-parts":[[2003,3,24]],"date-time":"2003-03-24T06:58:46Z","timestamp":1048489126000},"page":"646-653","source":"Crossref","is-referenced-by-count":117,"title":["Analysis of native and chemically modified oligonucleotides by tandem ion\u2010pair reversed\u2010phase high\u2010performance liquid chromatography\/electrospray ionization mass spectrometry"],"prefix":"10.1002","volume":"17","author":[{"given":"Kenneth J.","family":"Fountain","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Gilar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Gebler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2003,2,18]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/mas.10011"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02921611"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(00)00521-5"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00171a028"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00024a716"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac00280a023"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1089\/oli.1.1997.7.13"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5366.1077"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/bms.1200230604"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)00833-0"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac990378j"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(99)00953-X"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960557q"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0896(98)00098-4"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-9888(199703)32:3<305::AID-JMS482>3.0.CO;2-R"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)93430-S"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02278754"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290090121"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290090102"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac980791b"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290091007"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac960916h"},{"key":"e_1_2_5_24_2","unstructured":"GilarM WheatTE ChumsaeC BudmanY BouvierESP.HPLC 2000 Seattle WA USA2000; Poster 1112."},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.2001.5386"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(97)00430-1"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(02)00306-0"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(87)90288-0"},{"key":"e_1_2_5_29_2","first-page":"136","volume":"19","author":"Ausserer WA","year":"1995","journal-title":"Biotechniques"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-4347(99)00571-X"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Frcm.959","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.959","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T13:21:20Z","timestamp":1761484880000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.959"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,2,18]]},"references-count":29,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2003,4,15]]}},"alternative-id":["10.1002\/rcm.959"],"URL":"https:\/\/doi.org\/10.1002\/rcm.959","archive":["Portico"],"relation":{},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,2,18]]}}}