{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T04:27:49Z","timestamp":1787891269264,"version":"build-2784847793"},"reference-count":47,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T00:00:00Z","timestamp":1697155200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81920108033"],"award-info":[{"award-number":["81920108033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074011"],"award-info":[{"award-number":["82074011"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82130115"],"award-info":[{"award-number":["82130115"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2023,12,30]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The renin\u2013angiotensin system produces a series of biologically active angiotensin (Ang) peptides. These Ang peptides are the major regulators of blood pressure and Na homeostasis, and play a critical role in maintaining cardiovascular and fluid homeostasis. The concentration of Ang peptides in the body is at trace levels, making their detection and quantification a challenge. In this study, a rapid and sensitive analytical method using mass spectrometry coupled with ultrahigh\u2010performance liquid chromatography (UHPLC\/MS) was developed to simultaneously quantify 14 Ang peptides.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>UHPLC\/MS was employed to quantify 14 Ang peptides in mouse and human plasma. An HSS T3 column (2.1\u2009\u00d7\u2009100\u2009mm, 1.8\u00a0\u03bcm) with an HSS T3 precolumn and triple\u2010quadrupole mass spectrometer combined with an electrospray ionization source were utilized. Sample pretreatment involved a one\u2010step protein precipitation using methanol. The total analysis time was within 7.5\u00a0min and the target peptides were detected in positive ion mode and quantified by selected reaction monitoring mode.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The method was validated for linearity, detection and quantification limits, precision, stability, recovery and matrix effect. The limits of detection of Ang II, Ang III, Ang\u2010(1\u20137), Ang\u2010(2\u20137), Ang\u2010(3\u20137), Ang\u2010(1\u20139), bradykinin, Asn\n                      <jats:sup>1<\/jats:sup>\n                      and Val\n                      <jats:sup>5<\/jats:sup>\n                      \u2010Ang II are all less than 1\u00a0pg\u2009mL\n                      <jats:sup>\u22121<\/jats:sup>\n                      , indicating high sensitivity. The intra\u2010day and inter\u2010day precision was within 15%, and the accuracy was between 85% and 115%. Meanwhile, the sample and reference solution were stable within 48\u2009h, and the recovery and matrix effect met the quantitative requirements.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The method is currently reported to allow the largest number of Ang peptide species to be detected at one time. In addition, the proposed method offers a fast and reliable approach for comprehensive analysis of Ang metabolism in biological samples, facilitating research on the physiological and pathological states of cardiovascular, kidney and respiratory diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9637","type":"journal-article","created":{"date-parts":[[2023,10,13]],"date-time":"2023-10-13T06:44:15Z","timestamp":1697179455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A comprehensive profiling of renin\u2013angiotensin system in mouse and human plasma by a rapid quantitative analysis of 14 angiotensin peptides using ultrahigh\u2010performance liquid chromatography with tandem mass spectrometry"],"prefix":"10.1002","volume":"37","author":[{"given":"Yamin","family":"Liu","sequence":"first","affiliation":[{"name":"MOE Key Laboratory of Standardization of Chinese Medicines and SATCM Key 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