{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:15:55Z","timestamp":1772118955422,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T00:00:00Z","timestamp":1699574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2020GXNSFBA297085"],"award-info":[{"award-number":["2020GXNSFBA297085"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004607","name":"Natural Science Foundation of Guangxi Province","doi-asserted-by":"publisher","award":["2023GXNSFBA026176"],"award-info":[{"award-number":["2023GXNSFBA026176"]}],"id":[{"id":"10.13039\/501100004607","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82204611"],"award-info":[{"award-number":["82204611"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2022MD723762"],"award-info":[{"award-number":["2022MD723762"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,1,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>\n                      Tetrandrine, the Q\u2010marker in\n                      <jats:italic>Stephaniae Tetrandrae<\/jats:italic>\n                      Radix, was proven to present an obvious antitumor effect. Until now, the metabolism and antitumor mechanism of tetrandrine have not been fully elucidated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      The metabolites of tetrandrine in rats were profiled using ultra\u2010high\u2010performance liquid chromatography coupled with time\u2010of\u2010flight mass spectrometry. The potential antitumor mechanism of tetrandrine\n                      <jats:italic>in vivo<\/jats:italic>\n                      was predicted using network pharmacology.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 30 metabolites were characterized in rats after ingestion of tetrandrine (10\u00a0mg\/kg), including 0 in plasma, 7 in urine, 11 in feces, 9 in liver, 8 in spleen, 4 in lung, 5 in kidney, 5 in heart, and 4 in brain. This study was the first to show the metabolic processes demethylation, hydroxylation, and carbonylation in tetrandrine. The pharmacology network results showed that tetrandrine and its metabolites could regulate AKT1, TNF, MMP9, MMP2, PAK1, and so on by involving in proteoglycan tumor pathway, PI3K\u2010Akt signaling pathway, tumor pathway, MAPK signaling pathway, and Rap1 signaling pathway.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The metabolism features of tetrandrine and its potential antitumor mechanism were summarized, providing data for further pharmacological validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9662","type":"journal-article","created":{"date-parts":[[2023,11,10]],"date-time":"2023-11-10T03:47:49Z","timestamp":1699588069000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Dissection of the antitumor mechanism of tetrandrine based on metabolite profiling and network pharmacology"],"prefix":"10.1002","volume":"38","author":[{"given":"Cheng\u2010Jun","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Science Guangxi Normal University  Guilin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Xin","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Science Guangxi Normal University  Guilin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong\u2010Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Waters Corporation Shanghai  Shanghai P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Shi","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Science Guangxi Normal University  Guilin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1255-4222","authenticated-orcid":false,"given":"Feng\u2010Xiang","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Collaborative Innovation Center for Guangxi Ethnic Medicine, School of Chemistry and Pharmaceutical Science Guangxi Normal University  Guilin P. R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,11,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2018.03.042"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440\u20101746.2008.05720.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1678\/j.0258\u2010879x.1992.03.022"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.11735\/j.issn.1671\u2010170X.2020.05.B007"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.13193\/j.issn.1673\u20107717.2023.03.054"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41401\u2010021\u201000693\u20106"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.16695\/j.cnki.1006\u20102947.2018.01.019"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.6039\/j.issn.1001\u20100408.2017.07.06"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.16016\/j.1000\u20105404.1988.02.001"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.13457\/j.cnki.jncm.2018.09.004"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2020.173575"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1000\u20109760.2012.05.001"},{"issue":"08","key":"e_1_2_8_14_1","first-page":"4750","article-title":"Efficacy mechanism of Danlou tablets in the treatment of phlegm and blood stasis syndrome in stable angina pectoris of coronary heart disease based on metabolomics","volume":"37","author":"Li Z","year":"2022","journal-title":"China J Traditional Chinese Med"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2019.01625"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2013.02.021"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1875\u20105364(21)60001\u20108"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2021.104636"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114615"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/4293265"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9205"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1039\/d0ra06382g"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.3194"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz382"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx1076"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1099"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.112128"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac194"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T12:51:51Z","timestamp":1761483111000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.9662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,11,10]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2024,1,15]]}},"alternative-id":["10.1002\/rcm.9662"],"URL":"https:\/\/doi.org\/10.1002\/rcm.9662","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.9662\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9662\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9662\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9662\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,11,10]]},"assertion":[{"value":"2023-07-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-10-05","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e9662"}}