{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:55:58Z","timestamp":1786978558950,"version":"build-2736575974"},"reference-count":16,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,1,25]],"date-time":"2024-01-25T00:00:00Z","timestamp":1706140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001962","name":"World Anti-Doping Agency","doi-asserted-by":"publisher","award":["19D07XD"],"award-info":[{"award-number":["19D07XD"]}],"id":[{"id":"10.13039\/501100001962","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,3,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>High\u2010resolution mass spectrometry (HRMS) has been demonstrated to be an alternative platform for quantitative analyses, identifying unknown compounds and gathering information for the elucidation of chemical structures. This work describes a method to detect 13 esters of testosterone (T) and 5 biomarkers in 0.1\u00a0mL of human serum using gas chromatography (GC) coupled to HRMS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Analytes were extracted from serum after deproteinization and liquid\u2013liquid extraction. The trimethylsilyl derivatives were analyzed using a gas chromatograph coupled to HRMS at low electron energy to minimize molecule fragmentation. The acquisition in profiling full\u2010scan mode was applied with a resolving power of 30\u2009000 at\n                      <jats:italic>m\/z<\/jats:italic>\n                      400. Linearity, lower limit of quantitation, and measurement uncertainty were assessed. Precision and accuracy were assessed at 0.5 and 2\u00a0ng\/mL, respectively. Mass accuracy (MA) and mass extraction window (MEW) were also evaluated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>T esters showed a linear response between 0.25 and 10\u00a0ng\/mL (except for undecanoate, enanthate, and propionate that showed lineal responses between 0.5 and 10\u00a0ng\/mL and isocaproate between 2 and 10\u00a0ng\/mL); detection limits remained between 0.1 and 0.5\u00a0ng\/mL and accuracy between 81% and 119%. The MA (MEW\u2009=\u200910\u00a0ppm) was maintained between \u22122.4 and 4.8\u00a0ppm. The biomarkers (T, androstenedione, dehydroepiandrosterone [DHEA], estradiol, and 17\u2010OH\u2010progesterone) showed a linear response within the evaluated range; quantification limits remained between 0.1 and 0.5\u00a0ng\/mL (except for DHEA), the accuracy between 88% and 99%, and precision between 3.5% and 10.8%. Measurement uncertainties were found between 5.6% and 17.2%. MA (MEW\u2009=\u20093\u00a0ppm) was maintained between \u22120.47 and 0.12\u2009ppm.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The method to detect T esters and five endogenous biomarkers in serum using GC coupled to HRMS showed linear responses up to 10\u00a0ng\/mL with adequate precision, accuracy, and uncertainties. It was possible to distinguish cholesterol from T\u2010isocaproate based on the MEW of 10\u00a0ppm, preventing false positives. In addition, this method allows searching for other biomarkers and\/or unknown metabolites and other ester forms not included here but at a later stage if necessary.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9685","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T02:30:35Z","timestamp":1706236235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Simultaneous quantification of endogenous biomarkers and detection of testosterone esters in human serum using gas chromatography\u2013high\u2010resolution mass spectrometry"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7066-4916","authenticated-orcid":false,"given":"Dayam\u00edn","family":"Mart\u00ednez_Brito","sequence":"first","affiliation":[{"name":"Laboratorio Antidoping FMSI, Federazione Medico Sportiva Italiana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8329-6596","authenticated-orcid":false,"given":"Patrizia","family":"Leogrande","sequence":"additional","affiliation":[{"name":"Laboratorio Antidoping FMSI, Federazione Medico Sportiva Italiana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9480-164X","authenticated-orcid":false,"given":"Daniel","family":"Jardines","sequence":"additional","affiliation":[{"name":"Laboratorio Antidoping FMSI, Federazione Medico Sportiva Italiana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8037-6750","authenticated-orcid":false,"given":"Xavier","family":"de la Torre","sequence":"additional","affiliation":[{"name":"Laboratorio Antidoping FMSI, Federazione Medico Sportiva Italiana  Rome Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5296-8126","authenticated-orcid":false,"given":"Francesco","family":"Botr\u00e8","sequence":"additional","affiliation":[{"name":"Laboratorio Antidoping FMSI, Federazione Medico Sportiva Italiana  Rome Italy"},{"name":"REDs\u2014Research and Expertise on Anti\u2010Doping Sciences, Institute of Sport Science University of Lausanne  Lausanne Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,1,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.9196"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1872\u20102040(19)61159\u2010X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470727140"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361\u2010018\u20102101\u20100"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2010.11.007"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5b04689"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.201701016"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2010.06.006"},{"key":"e_1_2_8_10_1","unstructured":"World Anti\u2010Doping Agency (WADA).Athlete Biological Passport Operating Guidelines;2023."},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhepr.2023.100756"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2020.122026"},{"key":"e_1_2_8_13_1","unstructured":"6PLUS1\u00ae Multilevel Serum Calibrator Set Steroid Panel 2. Chromsystems Instruments & Chemicals. Accessed November 13 2023.https:\/\/chromsystems.com\/en\/6plus1r-multilevel-serum-calibrator-set-masschromr-steroid-panel-2-72039.html"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/toxics10110687"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac302036r"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2017.10.042"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2019.460686"}],"container-title":["Rapid Communications in Mass Spectrometry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/rcm.9685","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T16:19:26Z","timestamp":1761495566000},"score":1,"resource":{"primary":{"URL":"https:\/\/analyticalsciencejournals.onlinelibrary.wiley.com\/doi\/10.1002\/rcm.9685"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,25]]},"references-count":16,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2024,3,30]]}},"alternative-id":["10.1002\/rcm.9685"],"URL":"https:\/\/doi.org\/10.1002\/rcm.9685","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.1002\/RCM.9685\/v2\/response1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9685\/v2\/decision1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9685\/v1\/review2","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9685\/v1\/review1","asserted-by":"object"},{"id-type":"doi","id":"10.1002\/RCM.9685\/v1\/decision1","asserted-by":"object"}]},"ISSN":["0951-4198","1097-0231"],"issn-type":[{"value":"0951-4198","type":"print"},{"value":"1097-0231","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,25]]},"assertion":[{"value":"2023-10-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-28","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e9685"}}