{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T11:13:46Z","timestamp":1770290026585,"version":"3.49.0"},"reference-count":36,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T00:00:00Z","timestamp":1712793600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82073956"],"award-info":[{"award-number":["82073956"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,6,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>In clinical diagnosis of liver injury, which is an important health concern, serum aminotransferase assays have been the go\u2010to method used worldwide. However, the measurement of serum enzyme activity has limitations, including inadequate disease specificity and enzyme specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>With the high selectivity and specificity provided by nano liquid chromatography\u2013tandem mass spectrometry (LC\/MS\/MS), this work describes a method for the simultaneous determination of six proteins in liver that can be potentially used as biomarkers for liver injury: glutamic\u2010pyruvic transaminase 1 (GPT1), glutamic oxaloacetic transaminase 1 (GOT1), methionine adenosyl transferase 1A (MAT1A), glutathione peroxidase 1 (GPX1), cytokeratin 18 (KRT18) and apolipoprotein E (APOE).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In validation, the method was shown to have good selectivity and sensitivity (limits of detection at pg\/mL level). The analytical method revealed that, compared with normal mice, in carbon tetrachloride\u2010induced acute liver injury mice, liver MAT1A and GPX1 were significantly lower (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01 and\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05, respectively), KRT18 was significantly higher (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05) and APOE and GPT1 were marginally significantly lower (\n                      <jats:italic>p<\/jats:italic>\n                      between 0.05 and 0.1). This is the first work reporting the absolute contents of GPT1, GOT1, MAT1A, GPX1 and KRT18 proteins based on LC\/MS.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The proposed method provides a basis for establishing more specific diagnostic indicators of liver injury.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9754","type":"journal-article","created":{"date-parts":[[2024,4,11]],"date-time":"2024-04-11T23:19:47Z","timestamp":1712877587000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous quantification of six proteins related to liver injury using nano liquid chromatography\u2013tandem mass spectrometry"],"prefix":"10.1002","volume":"38","author":[{"given":"Siyu","family":"Cai","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Su","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengtian","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science Zhejiang Chinese Medical University  Hangzhou China"},{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3669-6041","authenticated-orcid":false,"given":"David Da Yong","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of British Columbia  Vancouver BC Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3509-0686","authenticated-orcid":false,"given":"Binjun","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Chinese Academy of Sciences  Hangzhou 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