{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T20:22:46Z","timestamp":1790367766589,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T00:00:00Z","timestamp":1715644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100004325","name":"AstraZeneca","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004325","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000266","name":"Engineering and Physical Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000266","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,7,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Dispersity values are considered critical quality attributes for the quality control of poly(ethylene glycol) formulations due to the direct impact on drug performance. However, when these polymers are analysed using mass spectrometry, the design of the mass analyser can impact the oligomer response and affect the obtained dispersity values, so further understanding is needed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The deconvoluted electrospray ionisation mass spectra of poly(ethylene glycol)s obtained using supercritical fluid chromatography (SFC) hyphenated to different mass analysers were compared, and visualisation diagrams were used to understand the differences in the dispersity value calculations. Five calibration approaches based on a surrogate single oligomer that represents the whole distribution, or the whole distribution itself, for response selection, were used to evaluate ionisation efficiency prior to quantitation. The impact of using an internal standard (ISTD) on the expanded uncertainty was also assessed.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Although there were challenges related to the resolution of multiply charged species when low\u2010resolution instruments were used, similar quantitation capabilities were obtained to those when high\u2010resolution mass analysers were used. Evaluation of approaches using a surrogate oligomer or the whole distribution suggested the independence of both approaches and a constant ionisation efficiency across the oligomer chain length. The higher degree of chromatographic resolution of SFC allowed incorporating a monodispersed ISTD to improve the accuracy and precision of the method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The use of low resolution mass analysers was sufficient to provide accurate and precise dispersity values; however, higher resolution instruments were recommended for characterisation due to the improved mass resolution of ions. The introduction of a monodispersed ISTD improved precision without compromising the calculated dispersity value due to the lack of analyte suppression.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9765","type":"journal-article","created":{"date-parts":[[2024,5,14]],"date-time":"2024-05-14T04:30:09Z","timestamp":1715661009000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Dispersity determination of poly(ethylene glycol)s using supercritical fluid chromatography\u2010mass spectrometry and different mass analysers"],"prefix":"10.1002","volume":"38","author":[{"given":"Sergio","family":"Cancho\u2010Gonzalez","sequence":"first","affiliation":[{"name":"Faculty of Engineering and Physical Sciences, School of Chemistry University of Southampton  Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Ferguson","sequence":"additional","affiliation":[{"name":"New Modalities and Parenteral Development, Pharmaceutical Technology and Development, Operations AstraZeneca  Macclesfield UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julie M.","family":"Herniman","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Physical Sciences, School of Chemistry University of Southampton  Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. John","family":"Langley","sequence":"additional","affiliation":[{"name":"Faculty of Engineering and Physical Sciences, School of Chemistry University of Southampton  Southampton UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,5,14]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41551\u2010021\u201000698\u2010w"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.21276\/sajp.2016.5.7.7"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003088\u2010199427040\u201000004"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200902672"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1351\/pac197543010115"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0014\u20103057(96)00091\u20102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.1290070914"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021\u20109673(97)00549\u20109"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2020.461839"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr9901081"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202000768"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216\u2010002\u20101332\u20109"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/masy.19961100117"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.508"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2015.07.060"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2004.09.024"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13361\u2010010\u20100065\u20109"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/ma802199r"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00099a016"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.201300269"},{"key":"e_1_2_11_22_1","volume-title":"Electrospray Ionization Mass Spectrometry: Fundamentals, Instrumentation, and Applications","author":"Cole R","year":"1997"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1044\u20100305(97)00292\u20104"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jasms.2009.04.019"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.336"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/rcm.338"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2011.03.019"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202300425"},{"key":"e_1_2_11_29_1","unstructured":"Lingaityte D.Characterisation of complex MDI oligomers using supercritical fluid chromatography and mass spectrometry. 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