{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T13:07:30Z","timestamp":1793020050481,"version":"4.5.0"},"reference-count":61,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T00:00:00Z","timestamp":1717459200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,8,30]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Childhood precocious puberty (CPP) is a common pediatric endocrine disorder with significant associated risks. Zhibai Dihuang pill (ZBDHP), a classic recipe of the Qing dynasty with its efficacy of nourishing yin and clearing heat, can downregulate the expression of ESR1 in the uterus and ovaries, thereby inhibiting CPP. However, as of now, the main active ingredients and pharmacological mechanisms of ZBDHP remain unclear.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A comprehensive approach was proposed using ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole\u2010Exactive Orbitrap mass spectrometry (UHPLC\u2010Q\u2010Exactive Orbitrap\u2010MS) and network pharmacology to explore the potentially active constituents of ZBDHP and reveal the underlying mechanisms against CPP. Molecular docking was used to verify the possible mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 214 constituents derived were identified via UHPLC\u2010Q\u2010Exactive Orbitrap\u2010MS, and 12 of them were definitely characterized using reference standards. Subsequently, compounds tetrahydropalmatine, alisol C, 25\u2010anhydroalisol A 11\u2010acetate, hispidone, cavidine, alisol E, melianone, neogitogenin, denudatin B, and 16\u03b2\u2010hydroperoxyalisol B with related targets PIK3CA, HSD11B1, CYP19A1, AR, PTGS2, CDK2, NR3C1, MMP2, MMP1, and MAPK1 were regarded as key components and targets for ZBDHP treating CPP using the compound\u2013target\u2013pathway network. Besides, the results revealed that the pathways conduced obviously to therapeutic efficacy, including pathways in cancer, neuroactive ligand\u2013receptor interaction, and cyclic adenosine monophosphate\uff08cAMP\uff09 signaling pathways. Molecular docking indicated that PIK3CA, HSD11B1, and CYP19A1 exhibited high affinities to corresponding compounds. Overall, the study determined the multicomponent, multitarget, and multipathway mechanisms of ZBDHP against CPP.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study provided a new method for exploring the chemical constituents and pharmacology mechanism of traditional Chinese medicine.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9831","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T17:53:55Z","timestamp":1717523635000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Integrated multicomponent analysis based on ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole\u2010Exactive Orbitrap mass spectrometry and network pharmacology to elucidate the effective constituents and potential mechanism of Zhibai Dihuang pill in treating childhood precocious puberty"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4363-5466","authenticated-orcid":false,"given":"Jianguang","family":"Zhang","sequence":"first","affiliation":[{"name":"Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Southwest Minzu University  Chengdu China"},{"name":"Qinzhou Provincial Health School  Qinzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yang","sequence":"additional","affiliation":[{"name":"Tibetan Plateau Ethnic Medicinal Resources Protection and Utilization Key Laboratory of National Ethnic Affairs Commission of the People's Republic of China Southwest Minzu University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiansang","family":"Wulu","sequence":"additional","affiliation":[{"name":"Tibetan Plateau Ethnic 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