{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T02:29:58Z","timestamp":1785464998831,"version":"3.56.0"},"reference-count":28,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T00:00:00Z","timestamp":1726963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2024,9,22]],"date-time":"2024-09-22T00:00:00Z","timestamp":1726963200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["analyticalsciencejournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Rapid Comm Mass Spectrometry"],"published-print":{"date-parts":[[2024,12,15]]},"abstract":"<jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Phosphodiesterase 4 (PDE4) inhibitors are a newer class of drugs that induce bronchodilation and have anti\u2010inflammatory effects, making them susceptible to misuse as performance enhancers in competitive sports.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This study explores the metabolic conversion of PDE4 inhibitor ibudilast in thoroughbred horses after oral administration and in vitro using equine liver microsomes and\n                      <jats:italic>Cunninghamella elegans<\/jats:italic>\n                      . A liquid chromatography\u2010high resolution mass spectrometry method was used to postulate the plausible structures of the detected metabolites.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 20 in vivo metabolites were identified under experimental conditions, including 12 Phase I and 8 Phase II conjugated metabolites. Phase I metabolites were predominantly formed through hydroxylation (mono\u2010, di\u2010, and tri\u2010hydroxylation). Demethylated metabolites were also identified during this investigation. Additionally, the research detected Phase II metabolites conjugated with glucuronic and sulfonic acids.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The data presented here can assist in detecting the PDE4 inhibitor ibudilast and uncover its illicit use in competitive sports.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/rcm.9916","type":"journal-article","created":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T01:03:25Z","timestamp":1727053405000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Equine metabolic investigation of the phosphodiesterase\u20104 inhibitor ibudilast as a potential performance enhancer"],"prefix":"10.1002","volume":"38","author":[{"given":"Moses","family":"Philip","sequence":"first","affiliation":[{"name":"Equine Forensic Unit Central Veterinary Research 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